engleski

The Effect of ACE Inhibitor (perindopril) on Peroxidase Activity in vitro Conditions

Hypertension is one of the leading diseases of today. ACE inhibitors are used in the prevention and treatment of hyperthyroidism, as well as other diseases such as pulmonary hypertension, proteinuria, etc. In this study we examined the effect of ACE inhibitors (Perindopril) on peroxidase enzyme activity in in vitro conditions. By using electrochemical methods we have determined the type of inhibition. We have proven that prindopril has been noncompetitive bonded to the active site on the enzyme, but also on enzyme/substrate complex. From the Lineweaver-Burk diagram, we obtained the values of the maximum value of the current (I-max) and the Michaelis- Menten constant (K-m) without presence and in the presence of the perindopril inhibitor. Values of I-max vary depending on the concentration of perindopril, while the K-m values are slightly modified, which is confirmed by the theoretical explanation of the noncompetitive type of inhibition. With this work we came to the conclusion that ACE inhibitors can be linked to different sites on oxidoreductase and thus reduce oxidative stress in the organism.

Peroxidase ; enzyme kinetics ; perindopril ; electrochemical methods

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