engleski

Pomological and genetical analysis of three dogrose (Rosa canina L.) generative offsprings

Dog rose (Rosa canina L.) is a cosmopolitan plant species and a successful colonizer inhabiting a variety of habitats. Its phylogenetic development has not yet been completed and there are difficulties in labeling the species boundaries. Because of its nutritional value, it has potential for use in medicine, cosmetics and the food industry. Because of this, there is an interest in the development of varieties and hybrids and the increase of production in conventional and ecological farming. Three generative offsprings of three indigenous genotypes from different biogeographic regions of Croatia were analyzed on the most important pomology and genetic properties of dog roses. The same samples were analyzed by the molecular-genetic microsatellite (SSR) method to determine the genetic distance and the taxonomic status. The analysis used 23 SSR markers, six of which are descriptive. The used SSR markers and pomological analyzes will be used to determine the level of polymorphism and the degree of genetic distance between the genotypes. Different pomological properties such as fruit length, fruit width, single weight, single-flesh weight, fruit flesh count, shape index and total dry matter were measured on the genotypes of dog roses extracted from natural populations during two vegetation years. Molecular quantitative characteristics of the mentioned genotypes were analyzed by the methods of univariate statistics and presented by standard statistical indicators. For statistical analysis it was used SAS (SAS Institute Inc., 2004) computer program. Using the SSR markers and pomological analyzes, the taxonomic status of Croatian wild-type genotypes and the level of polymorphism and degree of genetic distance between Croatian populations were determined. Among the examined generative offsprings, the level of genetic variability for agronomically important properties was determined.

Rosa sp., SSR markers, taxonomy

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