engleski

Does photo-activated photosensitizer really contribute to root canal disinfection?

Background: The aim was to investigate the antibacterial activity of irradiation with high-power diode laser and irradiation with additional photosensitizer compared to conventional 2.5% sodium hypochlorite (NaOCl) irrigation during root canal treatment. Methods: 140 extracted premolar single-rooted teeth were inoculated with Enterococcus faecalis (ATCC 29212) or Streptococcus mitis (ATCC 49456) to obtain in vitro models of infected root canals. The two groups were further divided into 3 subgroups, depending on the antimicrobial treatment mode. High-power diode laser (power output 1.5 W, 810 nm) and dye application followed by HELBO laser irradiation according to the protocol for endodontics have been used. The cell viability was established using flow cytometry. Results: Growth of cells rinsed from bacterial biofilm was successfully confirmed with positive control on agar plates. A higher percent of dead S. mitis compared to E. faecalis was observed for all treatment methods. Irradiation with HELBO laser and photosensitizer was significantly more effective (p<0.001) compared to other treatments, followed by high-power diode laser irradiation and 2.5% NaOCl irrigation. Values were comparable for E. faecalis and S. mitis and proved that PDT had a greater antimicrobial effect proven by the higher percent of dead E. faecalis and S. mitis cells (p<0.001, respectively). Conclusions: The effect of diode laser on bacteria was negligible and could not completely eliminate bacterial cells. Application of diode laser could therefore be used only as an adjuvant method to the standard endodontic therapy. Nevertheless, PDT seemed to be an effective antibacterial method for infected root canals treatment in endodontics compared to hand instrumentation.

diode laser treatment, Enterococcus faecalis, root canal disinfection, photosensitizer, Streptococcus mitis

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