A Simple method for RNA isolation from formalin fixed and paraffin embedded lymphatic tissues (CROSBI ID 99371)
Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija
Podaci o odgovornosti
Korbler, Tajana ; Gršković, Marica ; Dominis, Marija ; Antica, Mariastefania
engleski
A Simple method for RNA isolation from formalin fixed and paraffin embedded lymphatic tissues
Gene activation that lies beneath lymphoid cell differentiation has been one of the most explored issues in immunology in the recent years. However, the analysis of this molecular event in lymphoproliferative diseases is often hampered by the lack of fresh material. Most tissues available for routine histological investigation are formalin fixed and paraffin embedded. Gene expression in such specimens could be analysed using reverse transcription of mRNA and polymerase chain reaction (RT-PCR). Therefore we adjusted and established a method for mRNA isolation from such specimens by a combination of previously reported protocols and a modification of the phenol/chloroform extraction method. Given the significance of transcription factors in the human hemopoietic system, we investigated whether mRNA could be successfully isolated from archival tissue, for a study on expression of Ikaros family transcription factors in lymphatic tissue. Although quantitative analysis of RNA isolated from archival tissue is probably not feasible due to the unpredictable degree of RNA isolation varying from sample to sample, we show here that screening analysis is possible and simple.
RNA isolation ; formalin fixed ; paraffin embedded ; lymph nodes ; archival material ; transcription factor
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Podaci o izdanju
74 (3)
2003.
336-340
objavljeno
0014-4800
1096-0945
10.1016/S0014-4800(03)00024-8
Povezanost rada
Biologija, Temeljne medicinske znanosti, Kliničke medicinske znanosti