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Pregled bibliografske jedinice broj: 110372

A Simple method for RNA isolation from formalin fixed and paraffin embedded lymphatic tissues


Korbler, Tajana; Gršković, Marica; Dominis, Marija; Antica, Mariastefania
A Simple method for RNA isolation from formalin fixed and paraffin embedded lymphatic tissues // Experimental and molecular pathology, 74 (2003), 3; 336-340 doi:10.1016/S0014-4800(03)00024-8 (međunarodna recenzija, članak, znanstveni)


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Naslov
A Simple method for RNA isolation from formalin fixed and paraffin embedded lymphatic tissues

Autori
Korbler, Tajana ; Gršković, Marica ; Dominis, Marija ; Antica, Mariastefania

Izvornik
Experimental and molecular pathology (0014-4800) 74 (2003), 3; 336-340

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
RNA isolation ; formalin fixed ; paraffin embedded ; lymph nodes ; archival material ; transcription factor

Sažetak
Gene activation that lies beneath lymphoid cell differentiation has been one of the most explored issues in immunology in the recent years. However, the analysis of this molecular event in lymphoproliferative diseases is often hampered by the lack of fresh material. Most tissues available for routine histological investigation are formalin fixed and paraffin embedded. Gene expression in such specimens could be analysed using reverse transcription of mRNA and polymerase chain reaction (RT-PCR). Therefore we adjusted and established a method for mRNA isolation from such specimens by a combination of previously reported protocols and a modification of the phenol/chloroform extraction method. Given the significance of transcription factors in the human hemopoietic system, we investigated whether mRNA could be successfully isolated from archival tissue, for a study on expression of Ikaros family transcription factors in lymphatic tissue. Although quantitative analysis of RNA isolated from archival tissue is probably not feasible due to the unpredictable degree of RNA isolation varying from sample to sample, we show here that screening analysis is possible and simple.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti, Kliničke medicinske znanosti



POVEZANOST RADA


Projekt / tema
0098090
0108107
0108126

Ustanove
Klinička bolnica "Merkur",
Institut "Ruđer Bošković", Zagreb,
Medicinski fakultet, Zagreb

Profili:

Avatar Url Tajana Korbler (autor)

Avatar Url Marija Dominis (autor)

Avatar Url Mariastefania Antica (autor)

Citiraj ovu publikaciju

Korbler, Tajana; Gršković, Marica; Dominis, Marija; Antica, Mariastefania
A Simple method for RNA isolation from formalin fixed and paraffin embedded lymphatic tissues // Experimental and molecular pathology, 74 (2003), 3; 336-340 doi:10.1016/S0014-4800(03)00024-8 (međunarodna recenzija, članak, znanstveni)
Korbler, T., Gršković, M., Dominis, M. & Antica, M. (2003) A Simple method for RNA isolation from formalin fixed and paraffin embedded lymphatic tissues. Experimental and molecular pathology, 74 (3), 336-340 doi:10.1016/S0014-4800(03)00024-8.
@article{article, year = {2003}, pages = {336-340}, DOI = {10.1016/S0014-4800(03)00024-8}, keywords = {RNA isolation, formalin fixed, paraffin embedded, lymph nodes, archival material, transcription factor}, journal = {Experimental and molecular pathology}, doi = {10.1016/S0014-4800(03)00024-8}, volume = {74}, number = {3}, issn = {0014-4800}, title = {A Simple method for RNA isolation from formalin fixed and paraffin embedded lymphatic tissues}, keyword = {RNA isolation, formalin fixed, paraffin embedded, lymph nodes, archival material, transcription factor} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


Uključenost u ostale bibliografske baze podataka:


  • SCIexp
  • EMBASE
  • EMBiology


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