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Pregled bibliografske jedinice broj: 1102038

Predominance and Diversity of GLRaV-3 in Native Vines of Mediterranean Croatia


Hančević, Katarina; Saldarelli, Pasquale; Čarija, Mate; Černi, Silvija; Zdunić, Goran; Mucalo, Ana; Radić, Tomislav
Predominance and Diversity of GLRaV-3 in Native Vines of Mediterranean Croatia // Plants, 10 (2021), 1; 17, 14 doi:10.3390/plants10010017 (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 1102038 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Predominance and Diversity of GLRaV-3 in Native Vines of Mediterranean Croatia

Autori
Hančević, Katarina ; Saldarelli, Pasquale ; Čarija, Mate ; Černi, Silvija ; Zdunić, Goran ; Mucalo, Ana ; Radić, Tomislav

Izvornik
Plants (2223-7747) 10 (2021), 1; 17, 14

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
grapevine viruses ; leafroll ; autochthonous cultivars ; screening ; molecular variants

Sažetak
Sixteen grapevine cultivars from Mediterranean Croatia were surveyed for the presence of 10 of the most economically important grapevine viruses. The presence of Grapevine fanleaf virus (GFLV), Arabis mosaic virus (ArMV), Grapevine leafroll associated virus-1, -2, and -3 (GLRaV-1 ; GLRaV-2 and GLRaV-3), Grapevine virus A (GVA) and B (GVB), Grapevine fleck virus (GFkV), Grapevine rupestris stem pitting associated virus (GRSPaV), and Grapevine Pinot gris virus (GPGV) were tested by reverse transcription polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA). All 71 analyzed clones were positive for the presence of one or more viruses. The most abundant one, detected in almost 95% of samples was GLRaV-3. In most of cases it was reported in mixed infections with GVA, GRSPaV, and GPGV. Virus genomes of GLRaV-3 infected vines were further characterized molecularly in order to determine their genetic diversity. Different genomic variants of heat shock 70 protein homologue (HSP70h) were identified by single- strand conformation polymorphism (SSCP) and sequenced. Sequence analysis confirmed their clustering into phylogenetic group I and/or phylogenetic group II. This study emphasizes the wide virus heterogenicity in Mediterranean vines and the predominant presence of GLRaV-3 phylogenetic groups I and II, either individually or in combination.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Poljoprivreda (agronomija), Biotehnologija, Interdisciplinarne biotehničke znanosti



POVEZANOST RADA


Projekti:
IP-2018-01-9622 - Patogeni potencijal Virusa uvijenosti lista vinove loze 3 i njegova interakcija s domaćinskim biljkama (GRAPE-PATH3) (Hančević, Katarina, HRZZ - 2018-01) ( POIROT)

Ustanove:
Institut za jadranske kulture i melioraciju krša, Split,
Prirodoslovno-matematički fakultet, Zagreb

Citiraj ovu publikaciju

Hančević, Katarina; Saldarelli, Pasquale; Čarija, Mate; Černi, Silvija; Zdunić, Goran; Mucalo, Ana; Radić, Tomislav
Predominance and Diversity of GLRaV-3 in Native Vines of Mediterranean Croatia // Plants, 10 (2021), 1; 17, 14 doi:10.3390/plants10010017 (međunarodna recenzija, članak, znanstveni)
Hančević, K., Saldarelli, P., Čarija, M., Černi, S., Zdunić, G., Mucalo, A. & Radić, T. (2021) Predominance and Diversity of GLRaV-3 in Native Vines of Mediterranean Croatia. Plants, 10 (1), 17, 14 doi:10.3390/plants10010017.
@article{article, year = {2021}, pages = {14}, DOI = {10.3390/plants10010017}, chapter = {17}, keywords = {grapevine viruses, leafroll, autochthonous cultivars, screening, molecular variants}, journal = {Plants}, doi = {10.3390/plants10010017}, volume = {10}, number = {1}, issn = {2223-7747}, title = {Predominance and Diversity of GLRaV-3 in Native Vines of Mediterranean Croatia}, keyword = {grapevine viruses, leafroll, autochthonous cultivars, screening, molecular variants}, chapternumber = {17} }
@article{article, year = {2021}, pages = {14}, DOI = {10.3390/plants10010017}, chapter = {17}, keywords = {grapevine viruses, leafroll, autochthonous cultivars, screening, molecular variants}, journal = {Plants}, doi = {10.3390/plants10010017}, volume = {10}, number = {1}, issn = {2223-7747}, title = {Predominance and Diversity of GLRaV-3 in Native Vines of Mediterranean Croatia}, keyword = {grapevine viruses, leafroll, autochthonous cultivars, screening, molecular variants}, chapternumber = {17} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus


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