Pretražite po imenu i prezimenu autora, mentora, urednika, prevoditelja

Napredna pretraga

Pregled bibliografske jedinice broj: 1102037

Characterization of Chitosan-Based Scaffolds Seeded with Sheep Nasal Chondrocytes for Cartilage Tissue Engineering


Rogina, Anamarija; Pušić, Maja; Štefan, Lucija; Ivković, Alan; Urlić, Inga; Ivanković, Marica; Ivanković, Hrvoje
Characterization of Chitosan-Based Scaffolds Seeded with Sheep Nasal Chondrocytes for Cartilage Tissue Engineering // Annals of biomedical engineering (2021) doi:10.1007/s10439-020-02712-9 (znanstveni, poslan)


CROSBI ID: 1102037 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Characterization of Chitosan-Based Scaffolds Seeded with Sheep Nasal Chondrocytes for Cartilage Tissue Engineering

Autori
Rogina, Anamarija ; Pušić, Maja ; Štefan, Lucija ; Ivković, Alan ; Urlić, Inga ; Ivanković, Marica ; Ivanković, Hrvoje

Vrsta, podvrsta
Radovi u časopisima, znanstveni

Izvornik
Annals of biomedical engineering (2021)

Status rada
Poslan

Ključne riječi
Chitosan ; Biodegradation ; Fibronectin ; Nasal chondrocytes ; Hyaline cartilage
(chitosan ; biodegradation ; fibronectin ; nasal chondrocytes ; hyaline cartilage)

Sažetak
The treatment of cartilage defect remains a challenging issue in clinical practice. Chitosan- based materials have been recognized as a suitable microenvironment for chondrocyte adhesion, proliferation and differentiation forming articular cartilage. The use of nasal chondrocytes to culture articular cartilage on an appropriate scaffold emerged as a promising novel strategy for cartilage regeneration. Beside excellent properties, chitosan lacks in biological activity, such as RGD-sequences. In this work, we have prepared pure and protein- modified chitosan scaffolds of different deacetylation degree and molecular weight as platforms for the culture of sheep nasal chondrocytes. Fibronectin (FN) was chosen as an adhesive protein for the improvement of chitosan bioactivity. Prepared scaffolds were characterised in terms of microstructure, physical and biodegradation properties, while FN interactions with different chitosans were investigated through adsorption–desorption studies. The results indicated faster enzymatic degradation of chitosan scaffolds with lower deacetylation degree, while better FN interactions with material were achieved on chitosan with higher number of amine groups. Histological and immunohistochemical analysis of in vitro engineered cartilage grafts showed presence of hyaline cartilage produced by nasal chondrocytes.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija, Temeljne tehničke znanosti, Interdisciplinarne tehničke znanosti



POVEZANOST RADA


Projekti:
IP-2014-09-3752 - Razvoj biokompatibilnih materijala na temelju hidroksiapatita za primjene u inženjerstvu koštanog tkiva (HATEA) (Ivanković, Hrvoje, HRZZ - 2014-09) ( POIROT)
EK-H2020-681103 - BIOengineered grafts for Cartilage Healing In Patients (BIO-CHIP) (BIO-CHIP) (Ježek, Davor; Marijanović, Inga; Matičić, Dražen, EK - H2020-PHC-2015-single-stage_RTD) ( POIROT)

Ustanove:
Medicinski fakultet, Zagreb,
Prirodoslovno-matematički fakultet, Zagreb,
Fakultet kemijskog inženjerstva i tehnologije, Zagreb

Citiraj ovu publikaciju

Rogina, Anamarija; Pušić, Maja; Štefan, Lucija; Ivković, Alan; Urlić, Inga; Ivanković, Marica; Ivanković, Hrvoje
Characterization of Chitosan-Based Scaffolds Seeded with Sheep Nasal Chondrocytes for Cartilage Tissue Engineering // Annals of biomedical engineering (2021) doi:10.1007/s10439-020-02712-9 (znanstveni, poslan)
Rogina, A., Pušić, M., Štefan, L., Ivković, A., Urlić, I., Ivanković, M. & Ivanković, H. (2021) Characterization of Chitosan-Based Scaffolds Seeded with Sheep Nasal Chondrocytes for Cartilage Tissue Engineering. Poslan u Annals of biomedical engineering. [Preprint] doi:10.1007/s10439-020-02712-9.
@unknown{unknown, year = {2021}, DOI = {10.1007/s10439-020-02712-9}, keywords = {Chitosan, Biodegradation, Fibronectin, Nasal chondrocytes, Hyaline cartilage}, journal = {Annals of biomedical engineering}, doi = {10.1007/s10439-020-02712-9}, title = {Characterization of Chitosan-Based Scaffolds Seeded with Sheep Nasal Chondrocytes for Cartilage Tissue Engineering}, keyword = {Chitosan, Biodegradation, Fibronectin, Nasal chondrocytes, Hyaline cartilage} }
@unknown{unknown, year = {2021}, DOI = {10.1007/s10439-020-02712-9}, keywords = {chitosan, biodegradation, fibronectin, nasal chondrocytes, hyaline cartilage}, journal = {Annals of biomedical engineering}, doi = {10.1007/s10439-020-02712-9}, title = {Characterization of Chitosan-Based Scaffolds Seeded with Sheep Nasal Chondrocytes for Cartilage Tissue Engineering}, keyword = {chitosan, biodegradation, fibronectin, nasal chondrocytes, hyaline cartilage} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


Citati





    Contrast
    Increase Font
    Decrease Font
    Dyslexic Font