Cytotoxic effect of ascorbic acid on osteosarcoma stem cells (CROSBI ID 698206)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | domaća recenzija
Podaci o odgovornosti
Antunović, Maja ; Skube, Karlo ; Caput Mihalić, Katarina ; Pušić, Maja ; Šimić Jovičić, Marijana ; Petrović, Tadija ; Kolundžić, Robert ; Marijanović, Inga
engleski
Cytotoxic effect of ascorbic acid on osteosarcoma stem cells
Osteosarcoma (OS) is a highly malignant bone tumor of children and young adults with poor survival rate. The cause lies in small population of chemotherapy-resistant cells, i.e. osteosarcoma stem cells (OS-SCs). Although ascorbic acid (AA) has long and controversial history of anti-cancer properties in vitro and in vivo, the mechanism of action has remained elusive especially in context of cancer stem cells. Thus, the aim of the study was to analyze AA as potential therapeutic for selective targeting of OS-SCs. Tumor tissue was enzymatically digested to isolate tumor cells, followed by sarcosphere assay to isolate OS-SCs, Osteosarcoma U2OS cell line, hMSCs and HEK 293 were used as control cell types. MTT assay was used to evaluate cytotoxic effect of AA (2.5 - 55 ug/ml) during 72h as well as relationship between cell number and AA concentration. While AA did not have any effect on hMSCs, U2OS and HEK 293, AA efficiently induced dose-dependent viability reduction of OS-SCs. lC50 values of AA increased with the number of seeded OS-SCs. Effect of AA on sarcosphere-forming ability was analyzed during 28 days showing successful reduction of sarcosphere formation after 6th day. AA-induced cell death type was determined by Annexin V/Pl staining showing that apoptosis was prevalent death mode with 7o% of Annexin V positive cells. Based on the obtained results, it can be concluded that AA has cytotoxic impact on OS-SCs.
ascorbic acid, osteosarcoma stem cells, cytotoxicity, apoptosis
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Podaci o prilogu
278-279.
2018.
objavljeno
Podaci o matičnoj publikaciji
Cytotoxic effect of ascorbic acid on osteosarcoma stem cells
Kružić, Petar ; Caput Mihalić, Katarina ; Gottstein, Sanja ; Pavoković, Dubravko ; Kučinić, Mladen
Zagreb:
1848-5553
Podaci o skupu
13. Hrvatski biološki kongres
poster
19.09.2018-29.09.2018
Poreč, Hrvatska
Povezanost rada
Biologija, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)