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Pregled bibliografske jedinice broj: 110096

The Usefulness of Cytogenetic Biomarkers in Assessment of Occupational Exposure to Microwave Radiation


Garaj-Vrhovac, Vera; Kopjar, Nevenka
The Usefulness of Cytogenetic Biomarkers in Assessment of Occupational Exposure to Microwave Radiation // Zbornik radova petog simpozija Hrvatskog društva za zaštitu od zračenja / Krajcar Bronić, Ines ; Miljanić, Saveta ; Obelić, Bogomil (ur.).
Zagreb, 2003. str. 369-375 (plenarno, međunarodna recenzija, cjeloviti rad (in extenso), znanstveni)


Naslov
The Usefulness of Cytogenetic Biomarkers in Assessment of Occupational Exposure to Microwave Radiation

Autori
Garaj-Vrhovac, Vera ; Kopjar, Nevenka

Vrsta, podvrsta i kategorija rada
Radovi u zbornicima skupova, cjeloviti rad (in extenso), znanstveni

Izvornik
Zbornik radova petog simpozija Hrvatskog društva za zaštitu od zračenja / Krajcar Bronić, Ines ; Miljanić, Saveta ; Obelić, Bogomil - Zagreb, 2003, 369-375

Skup
Simpozij Hrvatskog društva za zaštitu od zračenja (5 ; 2003)

Mjesto i datum
Stubičke Toplice, Hrvatska, 09.-11.04.2003

Vrsta sudjelovanja
Plenarno

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Radar-facility workers; occupational exposure; comet assay; chromatid breakage assay; micronucleus test

Sažetak
Cytogenetic biomarkers were applied and compared to investigate the genome damage in radar-facility workers daily exposed to microwave radiation and unexposed control subjects. The alkaline comet assay was used to evaluate primary DNA damage, while chromatid breakage assay (bleomycin sensitivity test) and micronucleus test were used to assess chromosomal senitivity and damage. Occupational exposure to microwave radiation correlated with an increase of genome damage in somatic cells. The overall levels of genome damage in exposed subjects were increased compared to control and showed interindividual variations. The extent of DNA migration was significantly increased in exposed subjects compared to control. After short exposure of cultured lymphocytes to bleomycin, cells of subjects occupationally exposed to microwave radiation responded with high numbers of chromatid breaks. The incidence and distribution of micronuclei in exposed subjects were also changed compared to control. Based on the results obtained, cytogentic biomarkers indicate that microwave radiation represents a potential DNA-damaging hazard. It was also confirmed that highly specific cytogenetic assays employed in the present study are hepful to identify occupationally exposed subjects with inborn genetic susceptibility and point out to the need of implementation of preventive measures in decreasing of risk at individual level.

Izvorni jezik
Engleski

Znanstvena područja
Biologija



POVEZANOST RADA


Projekt / tema
0022020

Ustanove
Institut za medicinska istraživanja i medicinu rada, Zagreb