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Comet assay application in cell genome damage evaluation due to ionizing radiation in vitro

The aim of the present study was estimation of the possible application of comet assay in DNA damage evaluation caused by different gamma radiation doses in peripheral human lymphocytes in vitro. Therefor whole blood samples of young healthy, non-smoking donors were taken. The samples were divided in 4 specimens. The first specimen was used as the control. Other three specimens were irradiated using constant gamma irradiation source (60Co) giving the dose rate of 0.907 cGy/s. Different specimens were irradiated for 51 s, 437 s and 1099 s, giving the doses of 0.5 Gy, 4 Gy and 10 Gy. In order to estimate dose-response curve, on the control and all 3 irradiated whole blood samples comet assay under alkali conditions was performed. The comet assay endpoints showed higher values for all irradiated blood samples compared to the control. For both, tail length and tail moment, dose-effect relationship was found to be linear-quadratic in a dose range of 0.5Gy and 10 Gy. By this work wee also pointed out possible usage of the comet assay in the detection of DNA lesions caused by extremely high radiation dose, which is not possible by using standard cytogenetic methods.

comet assay; gamma radiation; human lymphocytes; in vitro; dose-effect relationship

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