Pretražite po imenu i prezimenu autora, mentora, urednika, prevoditelja

Napredna pretraga

Pregled bibliografske jedinice broj: 1099825

Analysis of protein expression- possible panel for TGCT diagnostics


Raos, Dora; Krasić, Jure; Mašić, Silvija; Čorić, Marijana; Krušlin, Božo; Katušić Bojanac, Ana; Bulić- Jakuš, Floriana; Ježek, Davor; Ulamec, Monika; Sinčić, Nino
Analysis of protein expression- possible panel for TGCT diagnostics // Book of abstracts: HDBMBZO Crossroads in Life Sciences
Lovran, Croatia, 2019. str. 111-111 (poster, domaća recenzija, sažetak, znanstveni)


CROSBI ID: 1099825 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Analysis of protein expression- possible panel for TGCT diagnostics

Autori
Raos, Dora ; Krasić, Jure ; Mašić, Silvija ; Čorić, Marijana ; Krušlin, Božo ; Katušić Bojanac, Ana ; Bulić- Jakuš, Floriana ; Ježek, Davor ; Ulamec, Monika ; Sinčić, Nino

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of abstracts: HDBMBZO Crossroads in Life Sciences / - , 2019, 111-111

Skup
HDBMBZO Crossroads in Life Sciences. Congress of the Croatian Society of Biochemistry and Molecular Biology

Mjesto i datum
Lovran, Croatia, 25.-28.9.2019

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
Protein expression ; TGCT ; Diagnostic panel
(protein expression ; TGCT ; diagnostic panel)

Sažetak
Testicular germ cell tumor (TGCT) represents only 1% of all malignancies. However, it affects the young male population, thereby decreasing the quality of their reproductive health. TGCT is divided into seminomas(SE) and nonseminomas(NS). The Iatter group consists of four histologically different types, i.e. embryonal carcinoma (EC), teratoma (TE), yolk sac tumor (YST) and choriocarcinoma (CH). SE and NS, as well as types of NS, differ in their aggressiveness, therapy strategy and response to treatment, which makes a precise diagnosis crucial. In this study, protein expression of OCT4 was compared with SALL4. OCT4 is a marker already used in TGCT diagnostics, while SALL4 was reported as a novel sensitive marker. Therefore the aim was to analyze the expression of these genes in TGCT components, germ cell neoplasia in situ (GCNIS) and seminiferous tubules with preserved spermatogenesis (ST). In total 117 TGCT samples were cut to 4 pm slides and immunohistochemically analyzed. Morphometricanalysis of protein expression was performed by a pathologist and the results were statistically analyzed. Semi- quantification of protein expression was expressed by immunoreactivity score (IRS). To calculate diagnostic positivity a cut—off value of IRS was 4, i.e. IRS score 0—3 is considered negative, while IRS 24 was declared positive. The highest protein expression of both genes was detected in GCNIS. |n TGCT both OCT4 and SALL4 were expressed. Considering SE and NS, both genes were highly expressed in SE compared to whole NS group. Considering NS components, OCT4 was expressed only in EC. SALL4 was highly expressed in EC and YST while in CH and TE its expression was absent or low. Based on the obtained results, we are able to suggest a panel which could be used in the diagnosis of NS components. Joined protein expression of OCT4 and SALL4 differ between EC (OCT4+, SALL4 +) and YST (OCT4 -, SALL4+). The absence of SALL4 protein could point to CH and TE. In the differential diagnosis of SE and EC/YS those two markers alone are not helpfull. In conclusion, investigating protein expression ofjust one gene is not enough for diagnostic of either TGCT or TGCT components. However, protein expression analysis of SALL 4 and OCT 4 as a panel could have potential use in TGCT diagnostics.

Izvorni jezik
Engleski



POVEZANOST RADA



Citiraj ovu publikaciju

Raos, Dora; Krasić, Jure; Mašić, Silvija; Čorić, Marijana; Krušlin, Božo; Katušić Bojanac, Ana; Bulić- Jakuš, Floriana; Ježek, Davor; Ulamec, Monika; Sinčić, Nino
Analysis of protein expression- possible panel for TGCT diagnostics // Book of abstracts: HDBMBZO Crossroads in Life Sciences
Lovran, Croatia, 2019. str. 111-111 (poster, domaća recenzija, sažetak, znanstveni)
Raos, D., Krasić, J., Mašić, S., Čorić, M., Krušlin, B., Katušić Bojanac, A., Bulić- Jakuš, F., Ježek, D., Ulamec, M. & Sinčić, N. (2019) Analysis of protein expression- possible panel for TGCT diagnostics. U: Book of abstracts: HDBMBZO Crossroads in Life Sciences.
@article{article, year = {2019}, pages = {111-111}, keywords = {Protein expression, TGCT, Diagnostic panel}, title = {Analysis of protein expression- possible panel for TGCT diagnostics}, keyword = {Protein expression, TGCT, Diagnostic panel}, publisherplace = {Lovran, Croatia} }
@article{article, year = {2019}, pages = {111-111}, keywords = {protein expression, TGCT, diagnostic panel}, title = {Analysis of protein expression- possible panel for TGCT diagnostics}, keyword = {protein expression, TGCT, diagnostic panel}, publisherplace = {Lovran, Croatia} }




Contrast
Increase Font
Decrease Font
Dyslexic Font