engleski

Intermediate filaments in teratoma component of experimental mouse and human teratocarcinoma

Teratocarcinoma (TCa) is a type of testicular germ cell tumor that consists of embryonal carcinoma and teratoma. Teratocarcinoma can be obtained experimentally by transplanting 7.5 days old mouse egg cylinder beneath the kidney capsule. Histologically this experimental tumor look like human teratocarcinoma but molecular comparison of human and mouse experimental TCa have not been performed yet. We have already recorded that glia and neural tube were significantlymore frequent in mouse so we semi-quantified expression of intermediate filaments glial fibrillary acidic protein (GFAP) and neurofilament H (NFH) in human and mouse TCa to see if there will be any difference in expression of these proteins in different components. Twenty experimental mouse and twenty human TCa were formalin-fixed paraffin— embedded and slides were stained with antibodies against GFAP and NFH. Slides were semi— quantitatively analyzed and results were grouped regarding percentage of IHC-positively stained cells in four categories. Signal intensity was assessed as 0, 1, 2 or 3. IHC staining index (Sl) was calculated by multiplying the number of category regarding percentage of positively stained cells with number of staining intensity. GFAP showed Sl 9 in all experimental mouse TCa. Previously, on just one hematoxylin and eosin stained slide glia cells were noticed and just that sample had GFAP expression with Sl 9 while all other human slides had SI 0. Regarding NFH staining, glia cells showed high expression. NFH Sl of epithelial cells and connective tissue varied in both samples. HNF was highly expressed in situ component of human samples. All mouse ganglion cells and most mouse fat tissue showed high NFH expression while there was no ganglion cells or fat tissue present in human samples. Noteworthy, NFH consistently stained skeletal muscle in mouse sample but there was no skeletal muscle in human sample at all. Our results suggest that experimental mouse and human TCa do not diverse much in protein level of GFAP and NFH in components that are comparable. It is hard to make deduction of protein levels in components that are rare or nonexistent in one group of samples therefore sample size should be expanded.

intermediate filaments ; human teratocarcinoma ; mouse teratocarcinoma

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