miR-7 AND miR-34a sequence cloning and expression in a1235 glioblastoma cell line (CROSBI ID 287606)
Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija
Podaci o odgovornosti
Kolić, Dora ; Horvat, Luka ; Šetinc, Maja ; Antica, Mariastefania ; Matulić, Maja
engleski
miR-7 AND miR-34a sequence cloning and expression in a1235 glioblastoma cell line
miRNAs are small non-coding RNAs which have an important role in signalling circuits regulating different cell processes. miR-7 and miR-34a are known as tumour suppressors, and both of them can interfere with cell proliferation, differentiation, apoptosis and migration. We constructed plasmids containing pri-miRNA sequences for these two miRNAs and introduced them into the A1235 glioblastoma cell line. Clones containing increased expression of processed miR-7 and miR-34a were obtained. The proliferation and sensitivity to alkylation agent of transfected cells were similar to those of control cells. Our results indicate that an increase in miR-7 and miR34 expression alone in A1235 glioblastoma cells is not sufficient to change their proliferation or sensitivity to the influence of alkylating agents.
miR - 7 ; miR - 34a ; expression ; glioblastoma cell line
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
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Podaci o izdanju
3 (2)
2020.
31-38
objavljeno
2584-671X
10.33602/mebm.3.2.4