engleski

Expression of KIT gene in testicular seminoma

Testicular neoplasia represents only 1% of all neoplasia, but it affects young male population. Approximately 95 % of all testicular cancers are germ cell tumors. There are two groups of TGCT, seminoma and non-seminoma. Compared to non– seminoma, seminoma is hypomethylated. The hypomethylated genome is unstable and more exposed to mutations what brings to the emergence of tumor cells. Hypomethylated genes show higher expression rate than other genes. Consequently, the gene expression pattern between tumor and healthy tissue differs. Gene expression on the protein level is used for diagnosis of tumors. OCT 3⁄4 is a gene which is already used as a specific marker for the diagnosis of seminoma, but new markers could contribute to diagnostic protocols and patient management. Archive seminoma slices were collected. Immunohistochemical staining was done manually, on 5μm archive tissue sections in the Laboratory for Epigenetics and Molecular Medicine. To visualize our genes of interest, we used specific antibodies. Morphometric analysis of gene expression at the protein level was performed at University Hospital Sestre Milosrdnice Department of Clinical Pathology Ljudevit Jurak. Differential gene expression in tumor tissue and in healthy tissue was observed. Analyzing and quantifying expression of genes at the protein level can contribute to the development of new histopathological markers for seminoma in blood and semen.

kit- gene ; testicular seminoma

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