engleski

Comparison of the expression of c-KIT and OCT ¾ in testicular seminoma

Testicular germ cell tumours (TGCT) represent 95% of all testicular neoplasia. Although testicular neoplasia represents only 1% of all neoplasia, TGCT affect young male population thereby representing well recognized challenge in the frame of male reproductive health. TGCT are divided into to groups - seminoma and nonseminoma. OCT ¾ and c-KIT are used as a biomarkers for seminoma and embryonal carcinoma in routine diagnosis of TGCT. In this study, we wanted to compare the expression of c-KIT gene with the expression of OCT ¾ in seminoma tissue. 52 seminoma tissue were collected in this study, embedded in paraffin and cut to 5 µm slides which were immunohistochemically stained. For visualization of OCT ¾ and c-KIT we used specific antibodies. Patologist performed morphometric analysis for OCT ¾ and c-KIT expression. In all seminoma tissue c-KIT and OCT ¾ were expressed. Moreover, out of 52 tissue samples, in more then 50% of seminoma cells c-KIT was present in 34 patients while OCT ¾ in just 18 samples analysed. This study is a part of a bigger study in which we want to investigate different diagnostic, prognostic and possibly therapeutic biomarkers for testicular seminoma. According to our results, after the comparison of this two biomarkers which are already being used in the routine diagnosis for testicular seminoma, c-KIT has a higher specificity.

c-kit ; oct 3/4 ; testicular seminoma

nije evidentirano