engleski

Components comparison between human and experimental mouse teratocarcinoma

Testicular germ cell tumors are the most frequent malignancies among young male population. Teratocarcinoma (TCa) is a type of TGCT composed of teratoma, which consists of tissues derived from all the three germ layers, and embryonal carcinoma. This type of tumor can be obtained experimentally by transplanting 7.5 days old mouse egg cylinder beneath the kidney capsule. Although this experimental tumor histologically resembles human teratocarcinoma, detailed comparison of human and mouse experimental TCa have not been performed yet and therefore it is the aim of our study. Twenty experimental mouse and eighteen human TCa formalin-fixed paraffin-embedded tissues were stained with hemalaun and eosin and their components were determined. Statistical analysis was performed by the Fisher's exact test. Mouse experimental TCa is, in average, composed of more different components (6.95) than human TCa (4.33). Statistically significant difference was found in presence of cells of all four tissue types. Stratified squamous and columnar epithelium, skeletal muscle, adipose tissue, glia and neural tube were statistically significant more frequent in mouse. Our results suggest that experimentally produced TCa diverge from human TCa in tissue types they are constituted of. Further analysis of proliferative and apoptotic activity, pluripotency and various cell type differentiation markers with immunohistochemical method and capillary western blot are in progress.

human ; mouse ; teratocarcinoma

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