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PRIMJENA FTIR SPEKTROSKOPIJE I ANALIZE GLAVNIH KOMPONENATA U PROCJENI OKOLIŠNIH UTJECAJA NA BILJKE

In this study we used Fourier Transform Infrared (FTIR) Spectroscopy for detection of chemical changes in plant leaves under influence of different environmental factors. The FTIR spectra obtained from 60 dandelion (Taraxacum officinale Weber) samples collected at different locations (agricultural, non- agricultural, high traffic zones, exposed /not exposed to pollution, etc.) were compared. Dandelion has been chosen since, this plant is re-emerging as a food source not only for animals but also for humans. Also, it is considered as a good bioindicator of environmental pollution with PCBs, PAHs, dibenzofurans and metals. When we compared the FTIR spectra of dandelion samples collected from different locations, slightly differences were noticed in the values of maximum absorbance but greater were found in the peak positions. The most significant differences in the peaks position and the character of spectrum were in FTIR region between 900 cm-1 and 1200 cm-1, and around 2900 cm-1. PCA analysis showed that the location of a sample has substantial influence on the obtained FTIR results, so it is possible to distinguish the FTIR spectra of pollutined and non-polluted locations (i.e. control samples). Dandelion samples collected on different locations were successfully resolved and differentiated after chemometric treatments of PCA.

FTIR, dandelion, pollution, Principal Component Analysis

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