engleski

Cultivation of autologous limbal epithelial stem cells on amniotic membrane for human corneal regeneration

The Tissue and Cell Bank is a hospital unit of University Hospital Center “Sestre milosrdnice” in Zagreb that has laboratories with cleanrooms. The Tissue and cell bank, in daily routine work, is divided into tissue engineering and tissue banking. It also has the human resources required for donor evaluation, testing, processing, preservation, storage and distribution of human tissues for transplantation. Our Department of transfusion and regenerative medicine has established a unit for Quality Management that deals with aspects such as quality control in all segments of work in tissue banking and cell therapy. Cultivation of limbal stem cells is a well established method of cellular therapy for patients with limbal epithelial stem cell deficiency caused by chemical burns, thermal injuries or chronic immune inflammatory diseases. My presentation is going to focus on the analysis of human limbal cells and their characteristics evaluated for transplantation, followed by different kinds of methods like colony forming efficiency, flow cytometry, histopathology, immunocytochemistry, immunohistochemistry, florescence immunocytochemistry, ect. Growth media from limbal cells culture, were monitored for bacterial endotoxin before to clinical application.

autologous limbal epithelial stem cells, limbal graft, amniotic membrane

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