engleski

Determination of T2 and HT2 mycotoxins in cereals and cereal-based products using ELISA technique

The aim of this study was to determine the levels of T-2 and HT-2 mycotoxins in grain, mill products and cereal-based products from samples obtained for testing at the Teaching Institute of Public Health Dr. “Andrija Štampar” in Croatia. Twenty-nine samples of grain, mill products and cerealbased products, collected using random sampling method from trade turnover in Croatia, were tested for T2 and HT2 mycotoxins. Samples were prepared according to the manufacturer's kit Romer Labs, and after preparation, the amount of T2 and HT2 mycotoxins was determined by immuno-enzymatic method (ELISA). The absorbance of the prepared samples was recorded at 450nm. The limit of detection LOD is 29 ppb for corn and 57 ppb for barley. The applied method proved to be a quick and effective for the analyzed group of samples. The results ranged from 24.00 µg/kg to 104.50 µg/kg for cereal, and from 24.00 µg/kg to 333.10 µg/kg for products based on cereals, indicating considerable contamination. The results indicated the presence of studied mycotoxins in the Republic of Croatia, therefore, to get better and more accurate results, it is necessary to analyze a larger number of various types of samples, both grains and their products, all in order to protect human health.

T2 and HT2 ; mycotoxins ; ELISA

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