Electrochemical adhesion based differentiation of algal cell species (CROSBI ID 697068)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Novosel, Nives ; Ivošević DeNardis, Nadica
engleski
Electrochemical adhesion based differentiation of algal cell species
Fundamental understanding of adhesion behavior of algal cells at the model charged interface is of relevance for cell fusion, cell activity, and direct studies of non-specific interactions between living cells. Mercury electrode is a commonly used substrate for cell adhesion studies due to atomically smooth, chemically inert, conductive surface, and large set interfacial data. Attractive interaction between a single cell and the model charged interface is controlled by applied potentials using chronoamperometry. The adhesion of single cells causes a displacement of counter-ions from the electrical double layer. The instant flow of compensating current (amperometric signal) reflects the dynamics of adhesive contact formation and the spreading of a cell (1). We aim to examine the effect of cell barrier structure on adhesion and spreading of unicellular algal species at the model charged interface. As model organisms, four unicellular algal cell species were chosen. Results show that Dunaliella tertiolecta and Prorocentrum micans adhere to the species-specific potential range, and their amperometric signal amplitude is in line with cell size. Tetraselmis suecica and Cylindrotheca closterium do not adhere to the model charged interface behaving as an inert particle. The differences in adhesion behavior are in agreement with the reported mechanical properties of the cell barrier. Dunaliella cells are enclosed within a thin elastic plasma membrane with a glycocalyx surface coat and determined cell elasticity is in a tenth of kPa (2). Prorocentrum cells possess cellulose amphiesma. Tetraselmis cells are encased within a thin calcite incrustrated theca, while Cylindrotheca cells possess a rigid organosilicate cell wall with the elasticity up to 20 MPa (3). Electrochemical adhesion-based differentiation identified in the algal cell systems could be relevant for industrial processes enabling fast characterization of soft cell species and differentiation from the rigid cell species without sample pretreatment.
adhesion ; algae ; interface ; mechanical properties ; cell barrier
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Podaci o prilogu
112-112.
2020.
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objavljeno
Podaci o matičnoj publikaciji
The book of Elecnano9
Podaci o skupu
9th meeting of Electrochemistry in Nanoscience
predavanje
23.11.2020-24.11.2020
Pariz, Francuska
Povezanost rada
Biotehnologija, Interdisciplinarne prirodne znanosti, Kemija