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Electroactivated disposable pencil graphite electrode ‐ new, cost‐effective, and sensitive electrochemical detection of bioflavonoid hesperidin

Electroactivated disposable pencil graphite electrode (ePGE) was used for hesperidin detection with cv and dpv Electroactivation efficiency of the pencil graphite electrode (PGE) was examined with EIS and SECM. Enhancement of electron transfer kinetics after electroactivation was found. Hesperidin is irreversibly oxidized on ePGE (oxidation was the most pronounced at pH = 5.0). Linear dependence between the peak current and the hesperidin concentration from 5×10‐7 M to 1×10‐5 M was obtained. Determined LOD was 2×10‐7 M. Hesperidin was quantified in pharmaceutical formulation with ePGE and the results were in a good correlation with concentrations determined with HPLC‐DAD (R2 = 0.9462).

electroactivated disposable graphite electrode ; hesperidin ; SECM ; voltammetry

This research is supported by Anhui Provincial NaturalScience Foundation (Grants No. 2008085QB68 and No.1808085QB50), Natural Science Foundation of AnhuiProvincial Department of Education (No. KJ2019A0598),Excellent Young Talents Fund Program of Higher Educa-tion Institutions of Anhui Province (No. gxyq2019168),Foundation of State Key Laboratory of AnalyticalChemistry for Life Science (Grants No. SKLACLS2003)and Foundation of Henan Key Laboratory of Biomolecu-lar Recognition and Sensing (Grants No.HKLBRSK1905).