Quaternary ammonium salt based disinfectants reduce the expression of listeriolysin O in Listeria monocytogenes (CROSBI ID 696999)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Šrajer Gajdošik, Martina ; Begić, Marija ; Gašo- Sokač, Dajana ; Pavlović, Hrvoje ; Shevchuk, Olga ; Andjelković, Uroš ; Martinović, Tamara ; Josić, Djuro
engleski
Quaternary ammonium salt based disinfectants reduce the expression of listeriolysin O in Listeria monocytogenes
Bacterial resistance, caused among others by frequent use of disinfectants and antibiotics, has become one of the major problems in food industry. Therefore, better understanding of mechanisms of action of disinfectants is of great importance. Quaternary ammonium salts (QAC) have been extensively used as antimicrobial agents in food- processing industries and medicine. In this study, proteomic methods were used to identify changes in the proteome of foodborne pathogen Listeria monocytogenes after treatment with three QAC based disinfectants. LC-MS/MS analysis, followed by gene ontology searching, reveals disturbance in the synthesis of plasma membrane proteins and cell wall proteoglycans in treated samples. Down- regulation of some of key proteins from this group, that are important for bacterial growth, is also observed. Additionally, it is shown that Listeriolysin O (LLO), the major virulence factor of L. monocytogenes, is significantly down- regulated after treatment with each of the three investigated inhibitors. These results contribute to the already hypothesized mechanism of action of QAC-based inhibitors in Gram-positive bacteria that results in the disturbance of key cell surface proteins and proteoglycans. Furthermore, significant inhibition of Listeriolysin O suggests that this protein can be used as potential biomarker candidate for food contamination with L. monocytogenes.
foodborne pathogens, listeriolysin o, mechanism of action, proteome changes
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Podaci o prilogu
89-89.
2020.
objavljeno
Podaci o matičnoj publikaciji
Jukić, Ante
Zagreb : Osijek: Hrvatsko društvo kemijskih inženjera i tehnologa (HDKI) ; Prehrambeno tehnološki fakultet Sveučilišta Josipa Jurja Strossmayera u Osijeku
978-953-6894-75-8
Podaci o skupu
18. Ružičkini dani "Danas znanost - sutra industrija"
poster
16.09.2020-18.09.2020
Vukovar, Hrvatska