engleski

AIFM3 GENE EXPRESSION IN KIDNEYS OF YOTARI MICE

Aim of this study was to describe the spatial distribution differences of the expression pattern of AIFM3 protein between wild type (Dab1+/+), heterozygotes (Dab1+/- ) and yotari (Dab1 -/- ) mice with the intention to demonstrate the association of its potential roles in maintaining kidney function. Yotari mouse is designed with the disruption of proteininteracting (PI-PTB) domain by PGK-neo cassette. By mating Dab1 +/- we got three groups of pups, Dab1 -/- , Dab1 +/- and Dab1+/+ . At day four after birth the pups were sacrificed and the samples were embedded in paraffin. In the kidney sections of control and yotari mice the expression of AIFM3 protein was examined by immunofluorescence staining. We captured at least twenty images per different kidney structures (proximal convoluted tubules (PCT), distal convoluted tubules (DCT) and glomeruli) of each group of mice at 40× objective magnification. We marked positive any level of cytoplasmic staining. The percentage of AIFM3-immunoreactive cells was calculated and analysed in GraphPad Prism statistic program by 1way ANOVA test with the statistical significance of P<0.05. AIFM3 was expressed in all experimental groups with specific spatial expression pattern and distinct fluorescence intensity. Expression of marker in PCT in all groups was present, moderately in Dab1+/+ and highly in Dab1 -/- and Dab1 +/- group. There was no noticeable expression in glomeruli across all groups. Main difference was seen in the expression of marker in DCT. While in control group the expression was detectable in merely 29% of DCT, in the Dab1 -/- and Dab1 +/- group it was visible in about 50-53% of DCT (P<0.05). The difference in appearance of AIFM3 across the groups in the DCT indicates its potentially important role in the impairment of kidney function of Dab1 -/- and Dab1 +/- group of mice.

AIFM3, yotari mouse, kidney development

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