engleski

GENE EXPRESSION OF α-TUBULIN, INVERSIN AND DISHVELLED-1 IN POSTNATAL KIDNEY TISSUE

Microtubules are essential for ciliary architecture, mitosis, cell differentiation and polarity of the cell. Previously it has been suggested that primary cilium is involved in the nephrogenesis. α-tubulin is the part of the microtubule complex whereas Inversin forms complex with tubulin. This might play important role in kidney maturation. Inversin inhibits the canonical signal Wnt pathway by targeting cytoplasmic Dishvelled-1 (Dvl-1), also it plays the role as the switch between Wnt pathways. The aim of this study was to explore the expression of α-tubulin, Inversin and Dvl-1 in postnatal kidney development as the regular kidney maturation prolongs after birth into the early childhood. The expression of α-tubulin, Inversin and Dvl-1 was studied by double immunofluorescence on the paraffin sections of 1.5- and 7-year old human kidney tissues. The detected positive cells in proximal convoluted tubules (PCT), distal convoluted tubules (DCT) and in glomeruli were obtained by ImageJ software. The retrieved data were then analysed by Kruskall Wallis and Dunn tests. α-tubulin, Inversin and Dvl-1 were expressed in 1.5- and 7- year old kidneys with difference in model staining comparing kidney structures and maturity of the kidneys. α-tubulin had the highest expression in DCT of 7-year old kidneys in comparison with PCT and glomeruli of 1.5- and 7- year old kidney tissue (P<0.001). Inversin was highly expressed in glomeruli of 7-year old kidneys in comparison to PCT and DCT (P<0.0001). Glomeruli of 7-year old kidney tissue had much higher expression of Dvl-1 than 1.5-year old glomeruli (P<0.001). α-tubulin and Inversin expressed higher than Dvl-1 in PCT of 1.5-year old kidney (P<0.0001) and in glomeruli of 1.5- and 7-year old kidneys (P<0.001). α- tubulin, Inversin and Dvl-1 are expressed and co-localize during postnatal kidney development. Established pattern directs to possible involvement in regular kidney maturation.

α-TUBULIN, INVERSIN, DISHVELLED-1, kidney development

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