Niske doze gama zračenja smanjuju osjetljivost tumorskih stanica grlića maternice na cisplatinu (CROSBI ID 488868)
Prilog sa skupa u zborniku | izvorni znanstveni rad | domaća recenzija
Podaci o odgovornosti
Osmak, Maja ; Brozović, Anamaria
hrvatski
Niske doze gama zračenja smanjuju osjetljivost tumorskih stanica grlića maternice na cisplatinu
One of the major challenges of modern genetics is to apply recent advances in mutation research to improve the accuracy of the estimates of the genetic risk for humans. Because of the important implications for radiation protection, biological effects of low-dose radiation have been a focus of research in recent years. Previously we have found that human cervical carcinoma HeLa cells irradiated repeatedly with low doses of gamma rays (HeLa1500 cells) became resistant to cisplatin. In this study we examine whether this effect was caused by inhibition of apoptosis. In HeLa and HeLa1500 cells we determined the induction of apoptosis following the treatment with cisplatin (i) by counting apoptotic cells with characteristic morphological changes, (ii) by analysing the expression of apoptotic genes involved in cytochrome c/Apaf-1/caspase-9 and in Fas/FasL pathways by Western blot method, and (iii) by estimating the activities of caspases by commercial caspase detection kits. Our results show that low doses of gamma rays induced alterations in human cervical carcinoma cells that were reflected in inhibition of p53-independent cisplatin-induced apoptosis due to reduced activity of caspase 3.
gama zračenje; apoptoza; stanice
nije evidentirano
engleski
Low doses of gamma rays reduce the sensitivity of cervical carcinoma cells to cisplatin
nije evidentirano
gamma rays; apoptosis; cells
nije evidentirano
Podaci o prilogu
151-155-x.
2003.
objavljeno
Podaci o matičnoj publikaciji
Zbornik radova V. Simpozija Hrvatskog društva za zaštitu od zračenja
Krajcar-Bronić, Ines ; Miljanić, Saveta ; Obelić, Milko
Zagreb:
Podaci o skupu
V. Simpozij Hrvatskog društva za zaštitu od zračenja sa međunarodnim učešćem
predavanje
09.04.2003-11.04.2003
Stubičke Toplice, Hrvatska