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Pregled bibliografske jedinice broj: 109373

Niske doze gama zračenja smanjuju osjetljivost tumorskih stanica grlića maternice na cisplatinu


Osmak, Maja; Brozović, Anamaria
Niske doze gama zračenja smanjuju osjetljivost tumorskih stanica grlića maternice na cisplatinu // Zbornik radova V. Simpozija Hrvatskog društva za zaštitu od zračenja / Krajcar-Bronić, Ines ; Miljanić, Saveta ; Obelić, Milko (ur.).
Zagreb: Agraf Potočki Astra, Grafičke usluge, 2003. str. 151-155 (predavanje, domaća recenzija, cjeloviti rad (in extenso), znanstveni)


Naslov
Niske doze gama zračenja smanjuju osjetljivost tumorskih stanica grlića maternice na cisplatinu
(Low doses of gamma rays reduce the sensitivity of cervical carcinoma cells to cisplatin)

Autori
Osmak, Maja ; Brozović, Anamaria

Vrsta, podvrsta i kategorija rada
Radovi u zbornicima skupova, cjeloviti rad (in extenso), znanstveni

Izvornik
Zbornik radova V. Simpozija Hrvatskog društva za zaštitu od zračenja / Krajcar-Bronić, Ines ; Miljanić, Saveta ; Obelić, Milko - Zagreb : Agraf Potočki Astra, Grafičke usluge, 2003, 151-155

Skup
V. Simpozij Hrvatskog društva za zaštitu od zračenja sa međunarodnim učešćem

Mjesto i datum
Stubičke toplice, Hrvatska, 9-11.04.2003

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Domaća recenzija

Ključne riječi
Gama zračenje; apoptoza; stanice
(Gamma rays; apoptosis; cells)

Sažetak
One of the major challenges of modern genetics is to apply recent advances in mutation research to improve the accuracy of the estimates of the genetic risk for humans. Because of the important implications for radiation protection, biological effects of low-dose radiation have been a focus of research in recent years. Previously we have found that human cervical carcinoma HeLa cells irradiated repeatedly with low doses of gamma rays (HeLa1500 cells) became resistant to cisplatin. In this study we examine whether this effect was caused by inhibition of apoptosis. In HeLa and HeLa1500 cells we determined the induction of apoptosis following the treatment with cisplatin (i) by counting apoptotic cells with characteristic morphological changes, (ii) by analysing the expression of apoptotic genes involved in cytochrome c/Apaf-1/caspase-9 and in Fas/FasL pathways by Western blot method, and (iii) by estimating the activities of caspases by commercial caspase detection kits. Our results show that low doses of gamma rays induced alterations in human cervical carcinoma cells that were reflected in inhibition of p53-independent cisplatin-induced apoptosis due to reduced activity of caspase 3.

Izvorni jezik
Hrvatski

Znanstvena područja
Biologija



POVEZANOST RADA


Projekt / tema
0098076

Ustanove
Institut "Ruđer Bošković", Zagreb