Determination of volatile aromatic hydrocarbons and their degradation metabolites in human urine as an indicator of the state of the environment (CROSBI ID 696702)
Prilog sa skupa u zborniku | prošireni sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Aleksandar Bulog
engleski
Determination of volatile aromatic hydrocarbons and their degradation metabolites in human urine as an indicator of the state of the environment
This study covered the determination of benzene, toluene, ethylbenzene and xylene isomers as characteristic volatile aromatic hydrocarbons related to the environmental impact on human health.The chosen method for this research is the gas chromatography method based on solid phase analyte microextraction (HS-SPME).The study also reported a method to simultaneously determine S-phenyl mercapturic acid (S-PMA) and S-benzyl mercapturic acid(S-BMA) in human urine. Since constant exposure to benzene isomers it is necessary to quantify this issue. S-PMA is believed to be a sensitive and specific biomarker for evaluating benzene exposure at low levels but also a metabolite of sorbic acid. Specific metabolite of toluene is excreted in urine in the form of S-BMA. Urine samples were analyzed by ultra-highperformance liquid chromatography/tandem mass spectrometer in negative electrospray ionization mode. To perform matrix match calibration standards were prepared in synthetic urine. Target analytes eluted within 15 min in multiple reaction monitoring mode. In order to achieve maximum sensitivity instrument conditions were automatically optimized. Urine samples were refrigerated until analysis and stability was observed within 15 days. We can conclude that the selected methods represent a quality analytical tool for determining the concentrations of volatile aromatic hydrocarbons and their degradation metabolites in human urine.
Aromatic hydrocarbons, S-phenyl mercapturic acid, S-benzyl mercapturic acid
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Podaci o prilogu
17-17.
2020.
objavljeno
Podaci o matičnoj publikaciji
International days of public and environmental health profession 2020
Krulec A. ; Tajnikar S. ; Ovca A.
Ljubljana: Inštitut za sanitarno inženirstvo, Institute of Public and Environmental Health, Zaloška cesta 155, SI-1000 Ljubljana, Slovenija
978-961-94556-6-1
Podaci o skupu
International days of public and environmental health profession 2020
pozvano predavanje
22.10.2020-23.10.2020
Ljubljana, Slovenija