engleski

ANTIAGGREGATORY EFFECT OF PROPOLIS AND GINKGO BILOBA

Propolis and Ginkgo biloba extracts are often components of dietary supplements due to their beneficial effects on human health. Ginkgo biloba leaves extract contains phenolic acids, proanthocyanidins, flavonoids (e.g. isorhamnetin, kaempferol, quercetin) and the terpenoids (ginkgolides, bilobalides). These extracts are part of dietary supplements used for enhancement of cognitive functions. Propolis is resinous mixture and beneficial health effects such as antibacterial, antiviral, antitumoral, antiaggregatory, and hepatoprotective are mainly attributed to polyphenols – flavonoids and phenolic acids. The objective of this study was assessment of antiaggregatory potential of Ginkgo biloba and propolis extract on the platelet aggregation. Impedance aggregometry was performed on the whole blood samples of healthy subjects at the Croatian Institute of Transfusion Medicine. Adenosine diphosphate (ADP) and TRAP–hexapeptide surrogate of thrombin, were used as a weak and strong agonist of aggregation. In the ADP induced aggregation assay, propolis showed antiaggregatory effect at a concentration of 10 µM. In the same test, the antiplatelet effect of Ginkgo biloba extract was absent. In the TRAP aggregation test, propolis showed antiplatelet effect at a concentration of 20 µM as well as Ginkgo biloba extract. Combinations of propolis and ginkgo in a ratio of 1:25 and 1:50 showed an antiplatelet effect at 20 µM concentrations of extracts. Based on the results obtained, it can be concluded that the combination of propolis and Ginkgo biloba extracts in dietary supplements is justified from a pharmacodynamic aspect, as propolis and ginkgo have different mechanisms of action because they act on different signaling pathways of aggregation.

propolis, Ginkgo biloba, platelets, aggregation, impedance

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