engleski

Analysis of the cytokine levels in the cerebrospinal fluid of mild cognitive impairment and Alzheimer's disease patients

Alzheimer’s disease (AD) is characterized by elevated inflammation. The dysregulation of the inflammatory response is probably a better term because of the often-seen imbalance of immune mediators. The purpose of this preliminary study was to detect and see how certain CSF cytokine levels differ between AD, mild cognitive impairment (MCI) patients, and healthy controls (HC), and whether some groups of mediators are elevated in different stages of the disease. Using the Bio-Plex Pro Human Cytokine 48-Plex Screening Panel, we determined the levels of 25 cytokines in samples from 26 AD, 25 MCI patients, and 25 HC subjects. Out of 25 cytokines analyzed, nine of them (IL-8, IP-10, SCGF-b, SDF-1a, MCP-1, M-CSF, IL-3, IL-6, MIF) didn’t show a significant difference between groups, whereas all others were significantly elevated in AD patients (CTACK, GROa, IL-1α, HGF, IFN-γ, IL-1β, IL-7, IL-17A, SCF, IL-2Ra, IL-4, IL-13, IL-16, IL18, RANTES, TNF-α). Most of them belong to a group of pro-inflammatory factors, except HGF, IL-4, and IL-13. We hope that comparison of these findings with other reports will contribute to better understanding of the inflammation profile in AD and its prodromal stages.

Alzheimer's disease ; bead- and flow-based dual-laser multiplex detection instrument ; cerebrospinal fluid ; chemokines ; cytokines ; neuroflammation

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