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Pregled bibliografske jedinice broj: 1090983

Potential preanalytical and analytical vulnerabilities in the laboratory diagnosis of coronavirus disease 2019 (COVID-19)


Lippi, Giuseppe; Simundic, Ana-Maria; Plebani, Mario
Potential preanalytical and analytical vulnerabilities in the laboratory diagnosis of coronavirus disease 2019 (COVID-19) // Clinical chemistry and laboratory medicine, 58 (2020), 7; 1070-1076 doi:10.1515/cclm-2020-0285 (međunarodna recenzija, pregledni rad, znanstveni)


CROSBI ID: 1090983 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Potential preanalytical and analytical vulnerabilities in the laboratory diagnosis of coronavirus disease 2019 (COVID-19)

Autori
Lippi, Giuseppe ; Simundic, Ana-Maria ; Plebani, Mario

Izvornik
Clinical chemistry and laboratory medicine (1434-6621) 58 (2020), 7; 1070-1076

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, pregledni rad, znanstveni

Ključne riječi
COVID-19 ; coronavirus ; diagnosis ; reverse transcription polymerase chain reaction (RT-PCR)

Sažetak
Abstract A novel zoonotic coronavirus outbreak is spreading all over the world. This pandemic disease has now been defined as novel coronavirus disease 2019 (COVID-19), and is sustained by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). As the current gold standard for the etiological diagnosis of SARS-CoV-2 infection is (real time) reverse transcription polymerase chain reaction (rRT-PCR) on respiratory tract specimens, the diagnostic accuracy of this technique shall be considered a foremost prerequisite. Overall, potential RT-PCR vulnerabilities include general preanalytical issues such as identification problems, inadequate procedures for collection, handling, transport and storage of the swabs, collection of inappropriate or inadequate material (for quality or volume), presence of interfering substances, manual errors, as well as specific aspects such as sample contamination and testing patients receiving antiretroviral therapy. Some analytical problems may also contribute to jeopardize the diagnostic accuracy, including testing outside the diagnostic window, active viral recombination, use of inadequately validated assays, insufficient harmonization, instrument malfunctioning, along with other specific technical issues. Some practical indications can hence be identified for minimizing the risk of diagnostic errors, encompassing the improvement of diagnostic accuracy by combining clinical evidence with results of chest computed tomography (CT) and RT-PCR, interpretation of RT- PCR results according to epidemiologic, clinical and radiological factors, recollection and testing of upper (or lower) respiratory specimens in patients with negative RT-PCR test results and high suspicion or probability of infection, dissemination of clear instructions for specimen (especially swab) collection, management and storage, together with refinement of molecular target(s) and thorough compliance with analytical procedures, including quality assurance.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti



POVEZANOST RADA


Ustanove:
Farmaceutsko-biokemijski fakultet, Zagreb,
Klinička bolnica "Sveti Duh"

Profili:

Avatar Url Ana-Maria Šimundić (autor)

Citiraj ovu publikaciju

Lippi, Giuseppe; Simundic, Ana-Maria; Plebani, Mario
Potential preanalytical and analytical vulnerabilities in the laboratory diagnosis of coronavirus disease 2019 (COVID-19) // Clinical chemistry and laboratory medicine, 58 (2020), 7; 1070-1076 doi:10.1515/cclm-2020-0285 (međunarodna recenzija, pregledni rad, znanstveni)
Lippi, G., Simundic, A. & Plebani, M. (2020) Potential preanalytical and analytical vulnerabilities in the laboratory diagnosis of coronavirus disease 2019 (COVID-19). Clinical chemistry and laboratory medicine, 58 (7), 1070-1076 doi:10.1515/cclm-2020-0285.
@article{article, year = {2020}, pages = {1070-1076}, DOI = {10.1515/cclm-2020-0285}, keywords = {COVID-19, coronavirus, diagnosis, reverse transcription polymerase chain reaction (RT-PCR)}, journal = {Clinical chemistry and laboratory medicine}, doi = {10.1515/cclm-2020-0285}, volume = {58}, number = {7}, issn = {1434-6621}, title = {Potential preanalytical and analytical vulnerabilities in the laboratory diagnosis of coronavirus disease 2019 (COVID-19)}, keyword = {COVID-19, coronavirus, diagnosis, reverse transcription polymerase chain reaction (RT-PCR)} }
@article{article, year = {2020}, pages = {1070-1076}, DOI = {10.1515/cclm-2020-0285}, keywords = {COVID-19, coronavirus, diagnosis, reverse transcription polymerase chain reaction (RT-PCR)}, journal = {Clinical chemistry and laboratory medicine}, doi = {10.1515/cclm-2020-0285}, volume = {58}, number = {7}, issn = {1434-6621}, title = {Potential preanalytical and analytical vulnerabilities in the laboratory diagnosis of coronavirus disease 2019 (COVID-19)}, keyword = {COVID-19, coronavirus, diagnosis, reverse transcription polymerase chain reaction (RT-PCR)} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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