engleski

Changes of bacterial profile of dental biofilm during orthodontic treatment and influence of fluorides and chlorhexidine

Aim: The aim of this study aimed to investigate how nickel and titanium ions released due to corrosion of orthodontic appliance influence bacteria of dental biofilm. Additionally, is the bacterial profile changed by the use of fluorides and chlorhexidine (CHX) aimed to prevent gingivitis and caries. Subjects and methods: Sample consisted of 30 subjects in orthodontic treatment with fixed orthodontic appliance aged 13-42 years, 56% females. There were 10 subjects in each experimental group – exposed to saliva, chlohexidine (Curaspet ADS 212, Curaden, Italy) or fluorides (Mirafluor-K-gel, Hager Werken, Germany) for a month. Dental biofilm was collected before the start of orthodontic treatment (T1) and during active treatment (T2). Plaque and gingival indices and pH of biofilm were assessed. Real-time polymerase chain reaction was used to analyze total bacterial count and count of Steptococcus mutans, sorbinus and salivarius. Results: Dental biofilm before the start of orthodontic treatment has similar amount of S. mutans and salivarius and much less sorbinus. Orthodontic appliance tend to increase S. mutans and pH of biofilm. Fluorides do not reduce accumulation of biofilm during orthodontic treatment, but they change cariogenic potential by increasing S. salivarius and reducing mutans. CHX increases S. salivarius but it does not reduce mutans and sorbinus. Gingivitis does not change significantly in either group. Due to great interindividual variability differences are not statistically significant. Conclusion: Corrosion products from orthodontic appliance increase cariogenicity of dental biofilm. Fluorides tend to reduce cariogenicity of biofilm of patients during orthodontic treatment with fixed appliance. CHX does not reduce cariogenicity.

cariogenic bacteria ; dental biofilm ; fluorides ; oral antiseptic ; orthodontic appliance

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