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Analysis of S-Phenylmercapturic acid and S- Benzylmercapturic acid in human urine by Liquid chromatography coupled with tandem mass spectrometry

This study reported a method to simultaneously determine S-phenyl mercapturic acid (S-PMA) and S-benzyl mercapturic acid (S-BMA) in human urine.Since constant exposure to benzene isomers it is necessary to quantify this issue. S-PMA is believed to be a sensitive and specific biomarker for evaluating benzene exposure at low levels but also a metabolite of sorbic acid. Specific metabolite of toluene is excreted in urine in the form of S-BMA. Urine samples were analyzed by ultra-high performance liquid chromatography/tandem mass spectrometer in negative electrospray ionization mode. To perform matrix match calibration standards were prepared in synthetic urine. Target analytes eluted within 15 min in multiple reaction monitoring mode. In order to achieve maximum sensitivity instrument conditions were automatically optimized. Urine samples were refrigerated until analysis and stability was observed within 15 days. This study was made as a part of the scientific research projects of the university in Rijeka uniri-biomed-18-146 1292 i uniri-biomed-18-220-6107.

Liquid chromatography, S-Phenylmercapturic acid, S- Benzylmercapturic acid

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