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Pregled bibliografske jedinice broj: 1090195

Effects of extracellular Hsp70, lipopolysaccharide and lipoteichoic acid on human monocyte-derived macrophages and differentiated THP-1 cells


Hulina-Tomašković, Andrea; Somborac-Bačura, Anita; Grdić Rajković, Marija; Hlapčić, Iva; Bosnar, Martina; Rumora, Lada
Effects of extracellular Hsp70, lipopolysaccharide and lipoteichoic acid on human monocyte-derived macrophages and differentiated THP-1 cells // Biological chemistry, 401 (2020), 10; 1181-1190 doi:10.1515/hsz-2020-0138 (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 1090195 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Effects of extracellular Hsp70, lipopolysaccharide and lipoteichoic acid on human monocyte-derived macrophages and differentiated THP-1 cells

Autori
Hulina-Tomašković, Andrea ; Somborac-Bačura, Anita ; Grdić Rajković, Marija ; Hlapčić, Iva ; Bosnar, Martina ; Rumora, Lada

Izvornik
Biological chemistry (1431-6730) 401 (2020), 10; 1181-1190

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
extracellular Hsp70 ; LPS ; LTA ; monocyte-derived macrophages ; THP-1 cells

Sažetak
Extracellular Hsp70 (eHsp70) is elevated in blood of patients with inflammatory diseases (e.g. chronic obstructive pulmonary disease), prone to bacterial infections. It stimulates pro-inflammatory effects in cells via TLR2 and TLR4. The main hypothesis was that eHsp70 induces inflammation and alters inflammatory responses to lipopolysaccharide (LPS) and lipoteichoic acid (LTA). We assessed pro-inflammatory cytokines concentrations, TLR2, TLR4 and Hsp70 mRNA expressions, NF-κB and MAPKs activation, and viability after treatment of monocyte-derived macrophages (MDMs) and THP-1 cells with recombinant human (rh)Hsp70, LPS and LTA. rhHsp70, LPS, LTA and their combinations induced IL-6, IL-8 and TNF-α from MDMs. LPS, LTA, and combinations with rhHsp70 increased TLR2 and decreased TLR4 in both cells. rhHsp70 increased TLR2 and TLR4 in THP-1 cells, and TLR2 in MDMs. rhHsp70, LPS and their combinations up-regulated Hsp70 in MDMs, while down-regulated it in THP-1. LPS and its combinations with rhHsp70 lowered cell viability in THP-1 cells and activated caspases-8 and -9. Involvement of NF-κB and MAPKs in inflammatory and survival mechanisms stimulated by rhHsp70 was demonstrated. eHsp70 alone and in combination with bacterial components could provoke inflammation and affect viability of MDMs and THP-1 cells leading to disturbed inflammatory responses in diseases accompanied with bacterial colonisations or infections.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Farmacija



POVEZANOST RADA


Projekti:
IP-2014-09-1247 - Uloga stresnog proteina Hsp70 u imunosno-upalnom odgovoru kod kronične opstrukcijske plućne bolesti (Hsp70COPD) (HRZZ - 2014-09) ( POIROT)

Ustanove:
Farmaceutsko-biokemijski fakultet, Zagreb,
Fidelta d.o.o.

Citiraj ovu publikaciju

Hulina-Tomašković, Andrea; Somborac-Bačura, Anita; Grdić Rajković, Marija; Hlapčić, Iva; Bosnar, Martina; Rumora, Lada
Effects of extracellular Hsp70, lipopolysaccharide and lipoteichoic acid on human monocyte-derived macrophages and differentiated THP-1 cells // Biological chemistry, 401 (2020), 10; 1181-1190 doi:10.1515/hsz-2020-0138 (međunarodna recenzija, članak, znanstveni)
Hulina-Tomašković, A., Somborac-Bačura, A., Grdić Rajković, M., Hlapčić, I., Bosnar, M. & Rumora, L. (2020) Effects of extracellular Hsp70, lipopolysaccharide and lipoteichoic acid on human monocyte-derived macrophages and differentiated THP-1 cells. Biological chemistry, 401 (10), 1181-1190 doi:10.1515/hsz-2020-0138.
@article{article, year = {2020}, pages = {1181-1190}, DOI = {10.1515/hsz-2020-0138}, keywords = {extracellular Hsp70, LPS, LTA, monocyte-derived macrophages, THP-1 cells}, journal = {Biological chemistry}, doi = {10.1515/hsz-2020-0138}, volume = {401}, number = {10}, issn = {1431-6730}, title = {Effects of extracellular Hsp70, lipopolysaccharide and lipoteichoic acid on human monocyte-derived macrophages and differentiated THP-1 cells}, keyword = {extracellular Hsp70, LPS, LTA, monocyte-derived macrophages, THP-1 cells} }
@article{article, year = {2020}, pages = {1181-1190}, DOI = {10.1515/hsz-2020-0138}, keywords = {extracellular Hsp70, LPS, LTA, monocyte-derived macrophages, THP-1 cells}, journal = {Biological chemistry}, doi = {10.1515/hsz-2020-0138}, volume = {401}, number = {10}, issn = {1431-6730}, title = {Effects of extracellular Hsp70, lipopolysaccharide and lipoteichoic acid on human monocyte-derived macrophages and differentiated THP-1 cells}, keyword = {extracellular Hsp70, LPS, LTA, monocyte-derived macrophages, THP-1 cells} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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