engleski

Plant growth regulators profiles during Aleppo pine (Pinus halepensis) somatic embryogenesis initiation under abiotic stress

Somatic embryogenesis is a worldwide used technology tool that offers the capacity to produce a large number of plants and could be of use in large-scale propagation programs. At the same time, has been widely used as a model system for understanding the physiological and biochemical events involved in stress adaptation response. Many studies in conifers have shown that plant growth regulators have a crucial function, since phytohormones regulate a wide number of aspects of plant growth, development and physiology and they have been long considered to be connected with somatic plant yield and productivity. Distinct endogenous phytohormone profiles under stress conditions are involved in plant responses to abiotic stress and play essential roles during all stages of somatic embryogenesis and embryo production. Taking into account our experience in Pinus halepensis somatic embryogenesis and the capacity to modify the environmental conditions during the process, high temperatures (40, 50 and 60ºC) were tested during different incubation periods at initiation stage in order to analyze the phytohormone metabolism of embryonal masses developed after abiotic stress stimuli and to determine their role in the different response in the plants obtained months later.

abiotic stress, endogenous phytohormones, Pinus halepensis, plant growth regulators, somatic embryogenesis

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