engleski

Sustainable production of lipase from Thermomyces lanuginosus: process optimization and enzyme characterization

The lipase production process by Thermomyces lanuginosus solid-state cultivation on hull-less pumpkin oil pomace was studied. A reduced quadratic model of lipase production was proposed based on the optimization of five independent variables followed by a model validation with 95% of the percentage of accuracy. Maximum lipase production was obtained after 2 days of process duration with high volumetric and specific activity. The enzyme activity of 391 U gdb-1 proves that the developed process is highly efficient. Partial purification of the crude enzymatic extracts was investigated and highly concentrated lipase liquid preparation with the volumetric activity of 422 U cm-3 was achieved. The sustainability of the process was confirmed by a snapshot assessment using the green chemistry matrix of E-factor and process mass index, quoting on the mass efficiency. The new lipase process was in the range of the best white biotechnology processes, as per green chemistry metrics ranking.

lipase ; Thermomyces lanuginosus ; response surface methodology ; lipase purification ; green chemistry metrics

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