engleski

A New Proteinaceous Factor Participating in the Rat Ejaculate Coagulation - Identification, Isolation and Function

The coagulation of rat ejaculate has been described by Manyai et al. (1965). The coagulation is mediated by the action of vesiculase, an enzyme of the coagulating gland (Manyai, 1964), and by a glycoprotein fraction isolated from the Cowper's gland (Hart, 1968, 1969, 1970 ; Hart and Greenstein, 1968), on the basic (Notides and Williams-Ashman, 1967) and insoluble (Koren at al., 1975) proteins of the rat seminal vesicle secretion. It is believed that the copulatory plug, thus formed, prevents the back-flow of spermatozoa from the female reproductive tract (Gotterer et al., 1955). Ejaculate coagulation is also well pronounced in the guinea pig, but the coagulation of the seminal vesicle basic protein (BP) is not effected by Cowper's gland secretion (Hart and Greenstein, 1968) as in rats. Furthermore, the same authors (1964) showed that the removal of the ventral prostate, i.e., the source of vesiculase, did not influence the fertility of rats. This finding suggets that, besides the already known factors participating in the process of copulatory plug formation, there might be some others responsible for this phenomenon. In this paper evidence is presented that, in the rat, 1 additional and as yet unrecognized protein is involved in the in vitro coagulation of rat seminal vesicle proteins.

Rat BP-reactive protein

nije evidentirano