engleski

Granulysin expression in peripheral blood lymphocyte in women with knee osteoarthritis correlated with pain and restricted daily physical activities

Introduction: Patients with osteoarthritis (OA) of the knee have limited daily activities due to local pain which correlates well with the serum pro-inflammatory cytokine concentrations. Pro-inflammatory cytokines support granulysin (GNLY) expression. The aim of this study was to evaluate whether GNLY correlates with the pain and with the daily activities in patients with knee OA. Patients and methods: In women with knee OA (no. 16), who agreed to participate in the study, age, pain intensity by visual analogue scale and daily activities tested by a 6-minute walk were evaluated during clinical examination. The women donated 10 ml of peripheral venous blood for intracellular labelling of GNLY protein in T and NK cells by triple immunofluorescence and flow cytometry. C-reactive protein (CRP) and erythrocyte sedimentation rate (ESR) were also analysed. Results: Frequency of total GNLY+ cells in patients with OA did not correlate with CRP and ESR. Total GNLY+ cells, GNLY+ NK cells and GNLY+ T cells positively correlated with the pain intensity. The intensity of pain negatively correlated with the 6-minute walk test. Neither the pain, nor the 6-minute walk test were affected by age in our investigation group. Total GNLY+ cells, GNLY+ NK cells and GNLY+ T cells negatively correlated with the 6-minute walk test. Conclusion: The intracellular GNLY protein expression in the peripheral blood lymphocytes, as markers of inflammation independent of the CRP and ESR, contributes to the development of local pain and shortens the 6-minute walk distance in patients with knee OA.

Granulysin ; Lymphocyte ; Osteoarthritis ; Pain ; Physical activity

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