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Pregled bibliografske jedinice broj: 108837

Toxinogenicity of Aspergillus fumigatus and A. versicolor strains isolated from pulmonary aspergilloma


Pepeljnjak, Stjepan; Slobodnjak, Z.; Pavlović, Mladen
Toxinogenicity of Aspergillus fumigatus and A. versicolor strains isolated from pulmonary aspergilloma // Focus on fungal infections
Maui, Hawaii: Imedex, 2003. str. 18-18 (poster, međunarodna recenzija, sažetak, znanstveni)


CROSBI ID: 108837 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Toxinogenicity of Aspergillus fumigatus and A. versicolor strains isolated from pulmonary aspergilloma

Autori
Pepeljnjak, Stjepan ; Slobodnjak, Z. ; Pavlović, Mladen

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Focus on fungal infections / - Maui, Hawaii : Imedex, 2003, 18-18

Skup
Focus on fungal infections 13

Mjesto i datum
Maui, Hawaii, 19.-21.03.2002

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Aspergilloma; Fungus ball; Aspergillus fumigatus; aflatoxin

Sažetak
Pulmonary aspergilloma-secondary form of pulmonary aspergillosis, is caused mainly by Aspergillus species chronic colonisation of lung tissue. Some strains of Aspergillus species have ability to produce mycotoxins, and their role in development of aspergilloma is not well understood. The aim of this study was to determine toxinogenicity of Aspergillus species isolated from operated lung tissue (pulmonary lobes) in 12 cases (8 males and 4 females) of pulmonary aspergilloma. Defrost tissue samples were twice examined by aseptic inoculation on Sabouraud agar with streptomycin and penicillin (20:40), and incubation at 250C+2 for 7 days. Identification of Aspergillus species was done on basis of macro and microscopic subculture characteristics on Czapek agar according to keys (Thom and Raper, 1945 ; de Hoog et al., 2000). Toxinogenicity of isolated Aspergillus strains was examined in vitro on liquid yeast medium. One ml of suspension of conidia (10 8-1/mL) from each strain was inoculated in sterile liquid yeast medium (25 mL), and incubated at 25 0C for 14 days with daily shaking. Qualitative and quantitative analysis of mycotoxins were carried out by previously described multytoxin TLC method (Balzer et al., 1978), and by TLC method for detection of gliotoxin (Richard et al., 1996). Aspergillus species were found in 5 samples of lung tissue, including 3 strains of A. fumigatus and 2 strains of A. versicolor. In biosynthesis, A. fumigatus strains produced aflatoxin B1 (AFB1) in concentration range from 0, 0025 to 0, 145 ug/ml, and 2 strains produced AFB1 and AFG1 simultaneously, in range from 0, 033 to 0, 042 ug/ml of biomass. Gliotoxin production was not detected. Multytoxinogenicity of 2 A. versicolor strains was also established. These strains produced AFB1 (0, 0035-0, 0094 ug/ml), and sterigmatocystin (0, 5-6, 67 ug/ml). Mycotoxinogenicity of Aspergillus can be one of possible virulence factors responsible for development of pulmonary aspergilloma.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti, Veterinarska medicina



POVEZANOST RADA


Projekti:
0022006
0006641

Ustanove:
Farmaceutsko-biokemijski fakultet, Zagreb,
Institut za medicinska istraživanja i medicinu rada, Zagreb

Profili:

Avatar Url Stjepan Pepeljnjak (autor)

Avatar Url Mladen Pavlović (autor)


Citiraj ovu publikaciju:

Pepeljnjak, Stjepan; Slobodnjak, Z.; Pavlović, Mladen
Toxinogenicity of Aspergillus fumigatus and A. versicolor strains isolated from pulmonary aspergilloma // Focus on fungal infections
Maui, Hawaii: Imedex, 2003. str. 18-18 (poster, međunarodna recenzija, sažetak, znanstveni)
Pepeljnjak, S., Slobodnjak, Z. & Pavlović, M. (2003) Toxinogenicity of Aspergillus fumigatus and A. versicolor strains isolated from pulmonary aspergilloma. U: Focus on fungal infections.
@article{article, author = {Pepeljnjak, Stjepan and Slobodnjak, Z. and Pavlovi\'{c}, Mladen}, year = {2003}, pages = {18-18}, keywords = {Aspergilloma, Fungus ball, Aspergillus fumigatus, aflatoxin}, title = {Toxinogenicity of Aspergillus fumigatus and A. versicolor strains isolated from pulmonary aspergilloma}, keyword = {Aspergilloma, Fungus ball, Aspergillus fumigatus, aflatoxin}, publisher = {Imedex}, publisherplace = {Maui, Hawaii} }
@article{article, author = {Pepeljnjak, Stjepan and Slobodnjak, Z. and Pavlovi\'{c}, Mladen}, year = {2003}, pages = {18-18}, keywords = {Aspergilloma, Fungus ball, Aspergillus fumigatus, aflatoxin}, title = {Toxinogenicity of Aspergillus fumigatus and A. versicolor strains isolated from pulmonary aspergilloma}, keyword = {Aspergilloma, Fungus ball, Aspergillus fumigatus, aflatoxin}, publisher = {Imedex}, publisherplace = {Maui, Hawaii} }




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