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Influence of hydrogen sulfide on de-etiolation of wheatgrass (Triticum aestivum L.)

Hydrogen sulfide (H2S) as a component of cellular signal transduction is involved in a large number of physiological processes and responses to abiotic types of stress. It is known it increases photosynthesis by stimulating the carboxylation pathway of RuBISCO and increasing chlorophyll synthesis. The aim of the study was to determine the influence of sodium hydrogen sulfide (NaHS) and time of application on the physiological properties of etiolated wheatgrass plants. Wheatgrass was grown under controlled conditions for five days without light and then with a 12-hour photoperiod, watered for 3 days with 100, 200 and 500 mM of NaHS solutions. Plants were watered from 7th-9th, 10th-12th and 13th-15th day. The highest content of phenols (268.56 µgGA/100 mg FW), flavonoids (153.72 µgQC/100 mgFW) and hydrogen peroxide (9.617 nmol/gFW) was found in wheatgrass plants watered with 100 mM NaHS. The highest proline content and lipid peroxidation were found in plants at 500 mM NaHS. At 200 mM NaHS, the highest content of total chlorophyll (1.08 mg/gFW), vitamin C (0.897 mg/100gFW) and antioxidant activity (IC50% 13.837 mgFW) were determined. Watering period significantly influenced the examined physiological parameters, as well. The results show that H2S significantly affects the de-etiolation process in wheatgrass plants.

etiolation ; hydrogen sulfide ; wheat grass

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