Copper - Phospholipid Interaction at Cell Membrane Model Hydrophobic Surfaces (CROSBI ID 695505)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Mlakar, Marina ; Cuculić, Vlado ; Frka Milosavljević, Sanja ; Gašparović, Blaženka
engleski
Copper - Phospholipid Interaction at Cell Membrane Model Hydrophobic Surfaces
Interactions of micronutrient metal ions with cells, primarily membranes, in the marine environment are of particular interest as they are crucial for cell function maintenance. Micronutrient metal resources are frequently limited and marine organisms develop different mechanisms for their consumption, which mostly allude their complexation with organic ligands to make micronutrient bioavailable. It is highly important to understand possible mechanism of acquisition of micronutrients to the cells. The aim of this study was to examine copper (II) interaction mechanism with lipids as vital part of cell membrane, for the first time. Investigation of Cu (II) binding with natural lipid phosphatidylglycerol (PG) in aquatic solution is carried out by voltammetric measurements at the mercury drop electrode used as model for hydrophobic cell membrane. Results are supported by monolayer studies in a Langmuir trough and zeta-potential measurements by dynamic light scattering (DLS) in combination with an applied electic field (electrophoresis). Penetration of Cu2+ ions into the PG layer is enabled by formation of hydrophilic Cu2+ - Phenanthroline (Phen) complex in the subphase, followed by mixed ligand complex Cu-Phen-PG formation at the hydrophobic interface. Cu-Phen-PG was identified in pH range from 6 to 9. Stoichiometry of the complex ([PhenCuOHPG]), as well as its stability and kinetics were determined at the mercury drop electrode.
Copper ; phospholipid ; interaction ; model membrane
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Podaci o prilogu
1265-1265.
2018.
objavljeno
Podaci o matičnoj publikaciji
Podaci o skupu
2018 Ocean science meeting
poster
11.02.2018-16.02.2018
Portland (OR), Sjedinjene Američke Države