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Ceruloplasmin and haptoglobin in bovine follicular fluid

Follicular fluid (FF) proteins originate from blood plasma and theca, granulosa cells and oocyte. The FF proteins regulate follicular development and oocyte maturation. Among numerous functions, they are acute phase proteins (APP) and proteins with antioxidant properties. Ceruloplasmin and haptoglobin are APP and protect follicular cells from oxidative damage binding copper and haemoglobin. The aim of the study was to determine follicle size and estrous cycle phase related changes in concentration of ceruloplasmin and haptoglobin. For the better insight in non- protein antioxidative molecules, biological antioxidant potential (BAP) was determined. Oxidative processes intensity was examined by reactive oxygen metabolites (ROMs). The bovine ovaries were collected from naturally cycling animals at a local abattoir. The stage of estrous cycle (follicular or luteal) was identified according to presence or absence of the corpus luteum on the ovary. Follicular diameter was measured and follicles were classified according to diameter: small (≤5 mm ; N=18 luteal phase ; N=14 follicular phase), medium (6-10 mm ; N=14 luteal phase ; N=12 follicular phase) or large (>10 mm ; N=12 luteal phase ; N=14 follicular phase) follicles from ovaries in both follicular and luteal stage of estrous cycle. Ceruloplasmin and haptoglobin concentrations and biological antioxidant potential (BAP) and concentration of ROMs were analysed in FF punctured from ovaries in follicular and luteal phase of estrous cycle. Concentrations of BAP and haptoglobin significantly decrease from small to large follicles collected in both luteal and follicular phase. The highest concentration of ceruloplasmin in FF was determined in medium size follicles but changes was not statistically significant compare to small and large follicles. During folliculogenesis, in luteal phase follicles ROM concentration significantly decreased from small to large follicles. During folliculogenesis ceruloplasmin negatively correlate with haptoglobin but significant correlation was noted only in small follicles from luteal phase (r= - 0.70 ; p<0.013). Ceruloplasmin as protein with antioxidant capacity and BAP as a sum of uric acid, bilirubin, vitamin C and E antioxidant potential significantly corelated in FF of small follicles in follicular phase of estrous cycle (r=0.66 ; p<0.05). Results indicate similar antioxidative properties of bovine follicles during both luteal/follicular phases of estrous cycle. Ceruloplasmin and haptoglobin concentrations showed different trends during folliculogenesis and negative correlation indicates their specific functions in FF.

bovine follicular fluid, luteal phase, follicular phase, ceruloplasmin, haptoglobin

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