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izvor podataka: crosbi

Sequence conservation of the IDL regions and the acidic TIPs within eubacterial SSB proteins (CROSBI ID 695328)

Prilog sa skupa u zborniku | sažetak izlaganja sa skupa

Paradžik, Tina ; Filić, Želimira ; Vujaklija, Dušica Sequence conservation of the IDL regions and the acidic TIPs within eubacterial SSB proteins // Book of abstracts of Power of microbes in industry and environment 2019. 2019. str. 61-61

Podaci o odgovornosti

Paradžik, Tina ; Filić, Želimira ; Vujaklija, Dušica

engleski

Sequence conservation of the IDL regions and the acidic TIPs within eubacterial SSB proteins

SB (single-stranded DNA binding) proteins are essential in all domains of life. These proteins bind and protect single-stranded DNA (ssDNA) during DNA recombination, replication and repair. With some exceptions, the eubacterial SSB proteins act as a homotetramers. Each monomer has two distinct domains: N-terminal domain with a conserved oligonucleotide-oligosaccharide fold (OB-fold) responsible for ssDNA binding, and C-terminal domain (Ct) with conserved acidic tip (TIP). Ct is intrinsically disordered (IDL) and play important role in mediating SSB-protein interactions. Recently, rrepetitive motifs, PXXP and GGX, which contibutes to elastomeric properties were found within IDL regions of SSB proteins. The aims of this study were: (i) To analyse the conservation of PXXP and GGX elements in IDL regions with respect to GC content of bacterial SSBs as well as the conservation of the acidic TIPs, (ii) to examine the impact of increased glycine/proline ratio in IDLs on the variations of these motifs in actinobacterial SSBs. SSB sequences were collected from genomes covering a wide range of GC content from 14 bacterial phyla. The ML trees were constructed for SSB sequences and corresponding 16S rRNA genes. The amino acids (aa) content of OB folds and IDL regions and TIPs were subsequently analysed. Variability and the presence of PXXP and GGX motives within IDLs were inspected. SSB protein sequence analyses suggested a correlation between amino aa composition and GC content. We will show how two distinct domains of SSB exhibit significant difference to the expected aa composition. In line with this, we will discuss the conservation of PXXP and GGX motives in SSBs within analysed bacterial phyla and with the respect to GC content.

SSB ; IDL regions ; sequence ; bacteria

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Podaci o prilogu

61-61.

2019.

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objavljeno

Podaci o matičnoj publikaciji

Book of abstracts of Power of microbes in industry and environment 2019

Podaci o skupu

Power of Microbes 2019

poster

15.05.2019-18.05.2019

Sveti Martin na Muri, Hrvatska

Povezanost rada

Biologija