engleski

Thin layer chromatographical determination of metals used as antibacterial components on biodegradable polymers

Metal and metal oxide nanoparticles provide excellent antimicrobial, water resistance and protective properties so they are today one of the most important antimicrobial reagents in the textile industry. Therefore, extraordinary antimicrobial activity of metals and metal oxides receives significant global interest in the development of new products. The antimicrobial activity of the nanoparticles is the result of their very small diameters, which are far below the range of the microorganisms’ dimensions. Such nano-sized metals and metal oxides can interact with both bacterial surfaces and/or with the bacterial core, after they enter inside the bacterial cell showing activity against different microorganisms, including Gram-positive and Gram- negative bacteria, as well as on spores that are resistant to high temperature and high-pressure. Different biodegradable polymers containing nanoparticles in their surface coatings are used in many protective materials. In this work, thin layer chromatography (TLC) was applied as a simple, rapid and effective method for determination of metals in a form of metal ions which are used as efficient antibacterial compounds in protective coatings on biodegradable polymers. The development was performed by acetonitrile: hydrochloric acid: water= 72: 25: 23 (v/v), on thirty different stationary phases. For visualization, NH3 vapors and UV light (254 and 366 nm) were applied. Results proved that spots of different compounds (namely Ag, Al, Au, Co, Cr, Cu, Fe, Ni, Mn, Pb, Zn and others) can easily be determined. Proposed TLC method is therefore an excellent choice for identification and quantification of many metals used as antimicrobial compounds present in coatings on wide variety of different biodegradable polymers.

thin layer chromatography ; antibacterial coating ; biodegradable polymers ; nanoparticles

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