Advancements in recombinant technology for production of butyrylcholinesterase, a bioscavenger of nerve agents (CROSBI ID 284520)
Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija
Podaci o odgovornosti
Čadež, Tena ; Kovarik, Zrinka
engleski
Advancements in recombinant technology for production of butyrylcholinesterase, a bioscavenger of nerve agents
Butyrylcholinesterase (BChE) is a serine hydrolase present in plasma and other mammalian tissues. As a target of organophosphorous pesticides and warfare nerve agents, BChE acts as their stoichiometric bioscavenger. However, so far it has been a significant challenge to produce BChE at large scales and low cost. For decades, numerous research efforts have been directed first at isolation from human volunteers and later at production of BChE in eukaryotic and prokaryotic expression systems. In this review we focused on recent studies on recombinant BChE discussing reasons why the efficient, economically sensible expression system for recombinant BChE is hard to develop. We also bring the most recent advancements in the use of expression of human BChE in vivo as an effective prophylactic against organophosphate poisoning.
Cholinesterase ; AAV gene transfer ; expression systems ; N-glycosylation
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Podaci o izdanju
121-122 (1-2)
2020.
55-63
objavljeno
0031-5362
1849-0964
10.18054/pb.v121i1-2.10867
Povezanost rada
Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje), Interdisciplinarne biotehničke znanosti, Kemija