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Pregled bibliografske jedinice broj: 1084864

A multi-enzyme strategy for the production of a highly valuable lactonized statin side-chain precursor


Švarc, Anera; Findrik Blažević, Zvjezdana; Vasić-Rački, Ðurđa; Charnock, Simon J.; Vrsalović Presečki, Ana
A multi-enzyme strategy for the production of a highly valuable lactonized statin side-chain precursor // Chemical engineering research & design, 164 (2020), 35-45 doi:10.1016/j.cherd.2020.09.016 (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 1084864 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
A multi-enzyme strategy for the production of a highly valuable lactonized statin side-chain precursor

Autori
Švarc, Anera ; Findrik Blažević, Zvjezdana ; Vasić-Rački, Ðurđa ; Charnock, Simon J. ; Vrsalović Presečki, Ana

Izvornik
Chemical engineering research & design (0263-8762) 164 (2020); 35-45

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
DERA ; AlDH ; Enzyme stability ; Statin ; Multi-enzyme process ; Mathematical modelling

Sažetak
Statins are a group of drugs used for lowering cholesterol levels in the human bloodstream to prevent cardiovascular diseases and its synthesis is a challenge because of chemical and stereochemical requirements. In this work, a multi-enzyme one-pot sequential strategy was employed to obtain lactonized statin side-chain precursor and was compared to the two-pot process. The enzymatic production of this valuable product is rarely examined comparing to the traditional chemical methods. The examined cascade consisted of two consecutive reactions: lactol production by the 2-deoxyribose-5-phosphate aldolase (DERA)- catalysed double aldol addition of acetaldehyde and chloroacetaldehyde and its oxidation by the NAD-dependent aldehyde dehydrogenase (AlDH). NADH oxidase (NOX) was employed for the coenzyme regeneration. The insight in all reactions of the system was performed by enzyme kinetic and stability studies. Based on these data the mathematical models were developed and validated in a batch reactor. The oxidation was carried out using both purified (two-pot) and crude lactol (one-pot) obtained in the DERA-catalysed reaction. Better process metrics and greater stability of the AlDH was observed in one-pot system, thus demonstrating an additional benefit in favor of running multi-enzymatic (cascade) processes.

Izvorni jezik
Engleski

Znanstvena područja
Kemijsko inženjerstvo, Biotehnologija

Napomena
This work has received funding from the EU Horizon 2020 research and innovation
programme under grant agreement 635595 "CarbaZymes".



POVEZANOST RADA


Projekti:
EK-H2020-635595 - Sustainable industrial processes based on a C-C bond-forming enzyme platform (CARBAZYMES) (Findrik Blažević, Zvjezdana, EK - H2020-LEIT-BIO-2014-1) ( POIROT)

Ustanove:
Fakultet kemijskog inženjerstva i tehnologije, Zagreb,
Fidelta d.o.o.

Citiraj ovu publikaciju

Švarc, Anera; Findrik Blažević, Zvjezdana; Vasić-Rački, Ðurđa; Charnock, Simon J.; Vrsalović Presečki, Ana
A multi-enzyme strategy for the production of a highly valuable lactonized statin side-chain precursor // Chemical engineering research & design, 164 (2020), 35-45 doi:10.1016/j.cherd.2020.09.016 (međunarodna recenzija, članak, znanstveni)
Švarc, A., Findrik Blažević, Z., Vasić-Rački, Ð., Charnock, S. & Vrsalović Presečki, A. (2020) A multi-enzyme strategy for the production of a highly valuable lactonized statin side-chain precursor. Chemical engineering research & design, 164, 35-45 doi:10.1016/j.cherd.2020.09.016.
@article{article, year = {2020}, pages = {35-45}, DOI = {10.1016/j.cherd.2020.09.016}, keywords = {DERA, AlDH, Enzyme stability, Statin, Multi-enzyme process, Mathematical modelling}, journal = {Chemical engineering research and design}, doi = {10.1016/j.cherd.2020.09.016}, volume = {164}, issn = {0263-8762}, title = {A multi-enzyme strategy for the production of a highly valuable lactonized statin side-chain precursor}, keyword = {DERA, AlDH, Enzyme stability, Statin, Multi-enzyme process, Mathematical modelling} }
@article{article, year = {2020}, pages = {35-45}, DOI = {10.1016/j.cherd.2020.09.016}, keywords = {DERA, AlDH, Enzyme stability, Statin, Multi-enzyme process, Mathematical modelling}, journal = {Chemical engineering research and design}, doi = {10.1016/j.cherd.2020.09.016}, volume = {164}, issn = {0263-8762}, title = {A multi-enzyme strategy for the production of a highly valuable lactonized statin side-chain precursor}, keyword = {DERA, AlDH, Enzyme stability, Statin, Multi-enzyme process, Mathematical modelling} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus


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