Chronic neurodegeneration and glial response following repetitive mild traumatic brain injury in the mouse optic tract are not affected by TDP-43 proteinopathy (CROSBI ID 694842)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Dolenec Petra, Pilipović Kristina, Rajič Bumber Jelena, Janković Tamara, Gržeta Nika, Križ Jasna, Župan Gordana
engleski
Chronic neurodegeneration and glial response following repetitive mild traumatic brain injury in the mouse optic tract are not affected by TDP-43 proteinopathy
Aims: Preclinical and clinical studies suggest visual system vulnerability to damage following repetitive mild traumatic brain injury (rmTBI). Increasing evidence points to association between rmTBI and neurodegenerative diseases in which Tar DNA binding protein 43 (TDP-43) neuropathology was observed. The aim of this study was to investigate if rmTBI induces chronic neurodegeneration and glial response in the optic tracts of mice and does the genetic predisposition to TDP-43 proteinopathy influence it. Methods: Brain traumas were induced by the weight drop method, during five consecutive days, twice daily in wild type C57BL/6J (WT) and transgenic TDP-43G348C (TGTDP-43) adult mice. Mice of the control groups were anesthetized without receiving head impacts. Animals were sacrificed 6 months after the last procedure and their brains were prepared for the histological analyses. Fluoro-Jade C staining was used for detection of neurodegeneration, and immunofluorescent labeling of Iba1 and GFAP for microglia and astrocytes identification. Results: We found significantly increased Fluoro-Jade C intensity levels as well as the Iba1 and GFAP immunoreactivities in the optic tracts of traumatized mice of both genotypes in comparison to the related control groups, but the differences between the injured WT and TGTDP-43 animals were not detected. Conclusions: Our research suggests the presence of chronic degeneration and pronounced glial activity in the mouse optic tracts 6 months following rmTBI that was not additionally affected by genetically acquired TDP-43 dysregulation. This research was fully supported by the Croatian Science Foundation under project IP-2016-06-4602 to Ž.G.
repetitive traumatic brain injury, optic tract, neurodegeneration, glia, mouse
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Podaci o prilogu
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2020.
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objavljeno
Podaci o matičnoj publikaciji
FENS 2020 Virtual Forum of Neuroscience
Podaci o skupu
FENS 2020 Virtual Forum of Neuroscience
poster
11.07.2020-15.07.2020
Glasgow, Ujedinjeno Kraljevstvo