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Synthesis and cloning of 12 CMTI-I analogues for their expression in plants


Kereša, Snježana; Marchetti, Stefano; Pfeiffer, Antonella; Pavlina, Renata
Synthesis and cloning of 12 CMTI-I analogues for their expression in plants // Periodicum Biologorum, Vol 100, Supplement 1 / Vitale, Branko (ur.).
Zagreb: Hrvatsko prirodoslovno društvo, 1998. str. 34-35 (poster, domaća recenzija, sažetak, znanstveni)


Naslov
Synthesis and cloning of 12 CMTI-I analogues for their expression in plants

Autori
Kereša, Snježana ; Marchetti, Stefano ; Pfeiffer, Antonella ; Pavlina, Renata

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Periodicum Biologorum, Vol 100, Supplement 1 / Vitale, Branko - Zagreb : Hrvatsko prirodoslovno društvo, 1998, 34-35

Skup
First Congress of Croatian Geneticists with international participation

Mjesto i datum
Hvar, Hrvatska, 1-4. 06. 1998

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
proteinase inhibitors; artificial genes; gene cloning

Sažetak
Proteinase inhibitors play an important role in limiting and defining the range of insect pests potentially harmful to plants. The introduction of foreign proteinase inhibitor genes into agricultural crops can therefore reduce damage caused by insects. In spite of their small size, squash serine proteinase inhibitors are very strong inhibitors of bovine &#61538 ; -trypsin and their use against insect trypsins is thought to lead to some success. Among different squash inhibitor family members, CMTI-I (from Cucurbita maxima L.) has been the one most intensively studied and several biochemical and physical properties are known. In this work, 12 artificial genes that could render inhibitors sharing the CMTI-I skeleton, but having a different combination of amino acids in the reactive site and in a flanking region were synthesised using DNA polymerase I (Klenow fragment) on partially overlapping forward and reverse primers. Following treatment with Taq polymerase, genes were cloned in the phagemid vector pGEM-T ; after transformation of E. coli strain JM 101, their sequence was checked by automatic sequencer and found correct. Genes were then subcloned in the Agrobacterium plasmid vector pBI 121 in place of the uidA gene. Recombinant pBI 121 plasmids were finally transferred to A. tumefaciens strain EHA 105 by triparental mating. The last procedure made artificial genes available for plant cell transformation.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija



POVEZANOST RADA


Projekt / tema
178322

Ustanove
Agronomski fakultet, Zagreb

Citiraj ovu publikaciju

Kereša, Snježana; Marchetti, Stefano; Pfeiffer, Antonella; Pavlina, Renata
Synthesis and cloning of 12 CMTI-I analogues for their expression in plants // Periodicum Biologorum, Vol 100, Supplement 1 / Vitale, Branko (ur.).
Zagreb: Hrvatsko prirodoslovno društvo, 1998. str. 34-35 (poster, domaća recenzija, sažetak, znanstveni)
Kereša, S., Marchetti, S., Pfeiffer, A. & Pavlina, R. (1998) Synthesis and cloning of 12 CMTI-I analogues for their expression in plants. U: Vitale, B. (ur.)Periodicum Biologorum, Vol 100, Supplement 1.
@article{article, editor = {Vitale, B.}, year = {1998}, pages = {34-35}, keywords = {proteinase inhibitors, artificial genes, gene cloning}, title = {Synthesis and cloning of 12 CMTI-I analogues for their expression in plants}, keyword = {proteinase inhibitors, artificial genes, gene cloning}, publisher = {Hrvatsko prirodoslovno dru\v{s}tvo}, publisherplace = {Hvar, Hrvatska} }