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Characterization of newly-formed tissue after nasal septum cartilage biopsy (CROSBI ID 435626)

Ocjenski rad | diplomski rad

Brezak, Matea Characterization of newly-formed tissue after nasal septum cartilage biopsy / Marijanović, Inga (mentor); Pušić, Maja (neposredni voditelj). Zagreb, Prirodoslovno-matematički fakultet, Zagreb, . 2019

Podaci o odgovornosti

Brezak, Matea

Marijanović, Inga

Pušić, Maja

engleski

Characterization of newly-formed tissue after nasal septum cartilage biopsy

Tissue engineering has become one of the most studied fields with clinical application. When producing cartilage tissue in vitro, nasal septum biopsy is taken in order to harvest chondrocytes that will be seeded on scaffolds. Following this procedure, the nasal septum is left with a perforation that fills in over time. The newly- formed tissue may not possess functional properties characteristic for septum cartilage, whereas if the neocartilage forms it would enable chondrocyte reharvesting and confirm normal septum function. To this time, no one has addressed the issue of tissue formation in the place of the perforation on a large animal model. Therefore, in this study, newly-formed tissue was characterized using histological and immunohistochemical stainings including analysis of cartilage and perichondrium marker gene expression. Low regenerative potential of cartilage was confirmed as no formation of similar tissue was observed over time. Perichondral origin of newly-formed tissue was confirmed both histologically and through gene expression analysis. Additionally, high expression of COL 1, COL 4 and CRABP 1 genes indicate a significant role of outer perichondrium layer in tissue genesis.

gene expression, immunohistochemical analysis, nasal cartilage, perichondrium, tissue engineering

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Podaci o izdanju

29

12.02.2019.

obranjeno

Podaci o ustanovi koja je dodijelila akademski stupanj

Prirodoslovno-matematički fakultet, Zagreb

Zagreb

Povezanost rada

Biologija, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)

Poveznice