Characterization of newly-formed tissue after nasal septum cartilage biopsy (CROSBI ID 435626)
Ocjenski rad | diplomski rad
Podaci o odgovornosti
Brezak, Matea
Marijanović, Inga
Pušić, Maja
engleski
Characterization of newly-formed tissue after nasal septum cartilage biopsy
Tissue engineering has become one of the most studied fields with clinical application. When producing cartilage tissue in vitro, nasal septum biopsy is taken in order to harvest chondrocytes that will be seeded on scaffolds. Following this procedure, the nasal septum is left with a perforation that fills in over time. The newly- formed tissue may not possess functional properties characteristic for septum cartilage, whereas if the neocartilage forms it would enable chondrocyte reharvesting and confirm normal septum function. To this time, no one has addressed the issue of tissue formation in the place of the perforation on a large animal model. Therefore, in this study, newly-formed tissue was characterized using histological and immunohistochemical stainings including analysis of cartilage and perichondrium marker gene expression. Low regenerative potential of cartilage was confirmed as no formation of similar tissue was observed over time. Perichondral origin of newly-formed tissue was confirmed both histologically and through gene expression analysis. Additionally, high expression of COL 1, COL 4 and CRABP 1 genes indicate a significant role of outer perichondrium layer in tissue genesis.
gene expression, immunohistochemical analysis, nasal cartilage, perichondrium, tissue engineering
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Podaci o izdanju
29
12.02.2019.
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Podaci o ustanovi koja je dodijelila akademski stupanj
Prirodoslovno-matematički fakultet, Zagreb
Zagreb
Povezanost rada
Biologija, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)