engleski

3D tissue engineered cartilage grafts for the repair of patellofemoral osteochondral kissing lesions

Osteochondral injuries include damage of articular cartilage and adjacent subchondral bone. They can occur in any joint but are most common in the knee. Osteochondral “kissing lesions˝ are still untreatable and can lead to the development of osteoarthritis. Kissing lesion in patellofemoral joint involves lesion on the patella and trochlea that are in the direct contact with each other. In our study we wanted to compare two types of autologous nasal chondrocyte-based tissue engineered cartilage grafts that are used for the treatment of patellofemoral osteochondral kissing lesion in a sheep model. Nasal chondrocytes (NC) were isolated from sheep nasal septum cartilage biopsy and expanded in monolayer for 13 days. Collagen scaffold (Chondro-Gide) was used to provide 3D environment for the cells. Two types of cartilage grafts were manufactured: N-CAM (immature graft with precultivation of 2 days) and N-TEC (mature grafts with pre-cultivation of 2 weeks). Manufactured grafts were embedded in paraffin and stained with HE, Safranin O and immunostained using antibodies directed against collagen type II and collagen type VI. N-TEC grafts contained higher amount of glycosaminoglycans (GAG) compared to the N-CAM grafts. Collagen type II was expressed only in N- TEC grafts. Collagen type VI was localized pericellulary in N-TEC grafts while in N-CAM grafts it was present throughout extracellular matrix.

kissing cartilage lesions, nasal chondrocytes, tissue engineering

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