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Iron oxide nanoparticles as a tool for MRI tracking of nasal chondrocytes (CROSBI ID 694510)

Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija

Pusic, Maja ; Marijanovic, Inga ; Skokic, Sinisa ; Horak, Daniel ; Gajovic, Srecko ; Vuckovic, Mirta ; Kostesic, Petar ; Maticic, Drazen ; Vnuk, Drazen ; Ivkovic, Alan Iron oxide nanoparticles as a tool for MRI tracking of nasal chondrocytes // Abstracts- 5th TERMIS World Congress. 2018. str. 636-636

Podaci o odgovornosti

Pusic, Maja ; Marijanovic, Inga ; Skokic, Sinisa ; Horak, Daniel ; Gajovic, Srecko ; Vuckovic, Mirta ; Kostesic, Petar ; Maticic, Drazen ; Vnuk, Drazen ; Ivkovic, Alan

engleski

Iron oxide nanoparticles as a tool for MRI tracking of nasal chondrocytes

Superparamagnetic iron oxide nanoparticles (SPION) can be used as contrast agents for tracking and visualization of cells and tissues with magnetic resonance imaging (MRI). In this study, we focused on two types of nanoparticles, D-mannose coated and PEG coated iron oxide nanoparticles. These nanoparticles showed low cytotoxicity properties and were detectable with MRI in previous studies. Since nasal chondrocytes (NC) are used in clinical study for treatment of articular cartilage defects, we wanted to examine the possibility of NC labelling with iron oxide nanoparticles and to assess their effect on chondrocyte viability and differentiation. Sheep nasal septum cartilage biopsies were dissected and digested in collagenase solution to obtain NC. Isolated chondrocytes were expanded in expansion medium until passage 2 and incubated with different concentrations of D-mannose γ- Fe2O3 and Fe3O4&SiO2-PEG nanoparticles for 1.5, 24, 48 and 72 h. Nanoparticle cytotoxicity was determined by Crystal violet staining method and Live Dead assay. In order to test chondrocytes differentiation potential in the presence of PEG and D-mannose coated nanoparticles, three dimensional pellet culture was established. Pellets were collected after 1, 7 and 14 days. For histological evaluation, pellets were stained with Safranin O and Prussian blue, and immunostained against collagen type II. Expression of collagen type I, II and aggrecan was determined by qPCR. To confirm detection of nanoparticles with MRI, T2 and T2* weighted sequences were obtained. Viability assays showed no significant reduction in chondrocyte viability after the treatment with D-mannose-coated and PEG-coated nanoparticles. Chondrocytes treated with D-mannose nanoparticles had higher proliferation rate than control cells or cells treated with PEGcoated nanoparticles. Histological staining and qPCR for chondrogenic markers confirmed secreted extracellular matrix in treated chondrocytes as in control cells. Prussian blue staining and MRI T2 and T2* maps confirmed presence of D-manose nanoparticles in pellets after 7 and 14 days. D-mannose coated iron oxide nanoparticles do not reduce viability and proliferation of NC. Their redifferentiation capacity also remained unaffected. Labeling efficiency of NC with D-mannose nanoparticles make them a potential agent for chondrocyte tracking after graft implantation.

iron oxide nanoparticles, cartilage, nasal chondrocytes, MRI tracking

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Podaci o prilogu

636-636.

2018.

objavljeno

Podaci o matičnoj publikaciji

Abstracts- 5th TERMIS World Congress

Podaci o skupu

5th TERMIS World Congress

poster

04.09.2018-07.09.2018

Kyoto, Japan

Povezanost rada

nije evidentirano