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Photodynamic activity of amphiphilic tripyridylporphyrins on melanoma cells

Melanomas are difficult to treat due to variety of resistance mechanisms, such as defects in apoptotic pathways, ABC transporters and melanin [1, 2]. Photodynamic therapy (PDT) shows some encouraging results against melanoma, thanks to multitarget mechanism of action [1]. Amphiphilic porphyrins have been widely investigated due to their possibility to pass through membrane, as well as being water soluble. Our research group has previously described activity of pyridylporphyrins on several cell lines, and amphiphilic porphyrins showed significantly higher PDT activity compared to their hydrophilic analogues [4]. Here we study the impact of conjugation of hydrophobic chains of various length within N- methylated and N-oxidised pyridylporphyrins. Physicochemical differences between groups are investigated on singlet oxygen production, stability, spectroscopic properties, and lipophilicity of the photosensitizers (PSs). Photodynamic activity will be evaluated on MEWO melanoma cell line by testing cytotoxicity and localization of the PSs inside cells.

photodynamic therapy ; amphiphilic porphyrins ; melanoma cell line

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