engleski

Gizzerosine-induced histopathological lesions in broiler chicks

1. The aim of this study was to investigate pathomorphological changes in broiler chicks fed with different doses of gizzerosine, a substance produced during the heat treatments of fish meal. 2. The experiment was carried out in Ross broiler chicks wich were divided into three groups: grouop A received 100% of non-medicated commercial mash for broiler chicks. During an experimental 5-d period, 50% of commercial mash was replaced with unheated fish meal (0.65 ppm gizzerosine) in group B and in grop C with heated fish meal (1.15 ppm gizzerosine). Fourteen chicks from each group were killed every day. Samples og gastrointestinal and lymphoid organs, lung, pancreas, liver, brain, and kidney tissue were sampled for histopathological analysis. Organs were embedded in parafin and stained with HE stain and using PAS reagent and Sudan 3 (frozen sections). 3. Necropsy did not revealed notable differences between treated groups. There were no significant histopathological changes in immunocompetent organs nor in the lungs, the pancreas, the kidney or the brain. Sharply demarcated multiple vacuoles were observed in the myocardium in group C toward the end of experiment. In group C the prevalent changes in the gizzard and the proventriculus were slight to severe cuticle erosions and edema of the lamina propria with or without multiple vacuoles, respectively, towards the end of experiment. The most prominent changes toward the end of experiment were dispersed cell vacuolisation in duodenal, jejunual, ilea and cecal lamina propria in group C. 4. In conclusion, it should be emphasised that extra-gizzard gizzerosine-induced lesions are probably not mediated by H2-receptor stimulation, but could be a consequence of cellular hypoxia.

nije evidentirano

nije evidentirano